Three-dimensional biological scaffold and method of making the same

• US 8,541,015 B1
• Filed: 07/27/2012
• Issued: 09/24/2013
• Est. Priority Date: 04/24/2008
• Status: Expired due to Fees

• Current Assignee: HRL Laboratories LLC (The Boeing Co.)
• Original Assignee: HRL Laboratories LLC (The Boeing Co.)
• Inventors: Kisailus, David, Jacobsen, Alan J., Zhou, Chaoyin
• Primary Examiner(s): Naff, David M
• Application Number:
  US13/560,932
• Time in Patent Office: 424 Days
• Field of Search: None
• US Class Current: 424/423
• CPC Class Codes: A61L 27/30 Inorganic materials
• CPC Class Codes: A61L 27/56 Porous materials, e.g. foams or sponges
• CPC Class Codes: C12M 25/14 Scaffolds; Matrices in general C12N5/0068

Abstract

A three-dimensional biological scaffold. The scaffold includes at least three sets of polymer waveguides extending along at least three respective directions. The at least three sets of polymer waveguides interpenetrate each other at a plurality of nodes to form a self-supporting structure. In some embodiments, the polymer waveguides may be bio-degradable. In still some embodiments, the three-dimensional biological scaffold may include one or more coating layers for covering surfaces of the polymer waveguides.

Claims

1. A method for forming a three-dimensional biological scaffold having a plurality of ordered interconnected pores, the method comprising:

placing a mask having a plurality of apertures between at least one collimated light source and a volume of photo-monomer, in a first position relative to the volume of photo-monomer;

directing a plurality of collimated light beams from the at least one collimated light source through the plurality of apertures into a portion of the volume of photo-monomer, while the mask remains in the first position relative to the volume of photo-monomer, to polymerize the portion to form a plurality of bio-degradable polymer waveguides; and

removing photo-monomer of the volume of photo-monomer not polymerized to leave behind the three-dimensional biological scaffold having a plurality of ordered interconnected pores,

wherein at least three of the plurality of bio-degradable polymer waveguides interpenetrate each other at an aperture of the plurality of apertures, and

wherein the quantity and opening sizes of the plurality of apertures are selected to adjust the quantity and dimensions of the plurality of ordered interconnected pores to facilitate ingress of at least one of biological cells, vascular tissue, or nutrient media.

2. The method of claim 1, wherein the plurality of ordered interconnected pores occupy a free space of not less than about 40% by volume and not greater than about 99% by volume of the three-dimensional biological scaffold.

3. The method of claim 1, wherein the plurality of ordered interconnected pores are sized to about 100 μ

m to 300 μ

m.

4. A method for forming a three-dimensional biological scaffold having a plurality of ordered interconnected pores, the method comprising:

placing a mask having a plurality of apertures between at least one collimated light source and a volume of photo-monomer, in a first position relative to the volume of photo-monomer;

directing a plurality of collimated light beams from the at least one collimated light source through the plurality of apertures into a portion of the volume of photo-monomer, while the mask remains in the first position relative to the volume of photo-monomer, to polymerize the portion to form a plurality of polymer waveguides;

removing photo-monomer of the volume of photo-monomer not polymerized to leave behind the three-dimensional biological scaffold having a plurality of ordered interconnected pores; and

covering surfaces of the plurality of polymer waveguides with at least one coating layer,

wherein at least three of the plurality of polymer waveguides interpenetrate each other at an aperture of the plurality of apertures, and

wherein the quantity and opening sizes of the plurality of apertures are selected to adjust the quantity and dimensions of the plurality of ordered interconnected pores to facilitate ingress of at least one of biological cells, vascular tissue, or nutrient media.

5. The method of claim 4, wherein the plurality of ordered interconnected pores occupy a free space of not less than about 40% by volume and not greater than about 99% by volume of the three-dimensional biological scaffold.

6. The method of claim 4, wherein the plurality of ordered interconnected pores are sized to about 100 μ

m to 300 μ

m.

7. The method of claim 4, wherein the at least one coating layer comprises one of metal or ceramic.