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Method for sequencing a polynucelotide template

  • US 8,541,172 B2
  • Filed: 04/12/2012
  • Issued: 09/24/2013
  • Est. Priority Date: 09/15/2009
  • Status: Active Grant
First Claim
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1. A method for sequencing nucleic acids, comprising(a) attaching a plurality of different nucleic acids to a surface;

  • (b) amplifying the different nucleic acids on the surface to form a plurality of clusters on the surface, wherein clusters in the plurality of clusters abut or overlap with each other on the surface, wherein each of the clusters comprises copies of a single nucleic acid from the plurality of different nucleic acids; and

    (c) determining the sequences of the amplified copies in the clusters, wherein the sequences are determined by a method comprising;

    (i) obtaining images of overlapping signals emanating from the clusters that abut or overlap with each other on the surface, and(ii) processing the images to assign nucleic acid sequence information to a single cluster, wherein one or more nucleic acids in a cluster are distinguished from surrounding nucleic acids in the same cluster.

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