Composition comprising a progenitor/precursor cell population

• US 8,541,232 B2
• Filed: 03/08/2007
• Issued: 09/24/2013
• Est. Priority Date: 03/08/2006
• Status: Active Grant

• Current Assignee: Kwalata Trading Ltd.
• Original Assignee: Kwalata Trading Ltd.
• Inventors: Porozov, Svetlana, Porat, Yael, Fulga, Valentin, Belleli, Adina
• Primary Examiner(s): Weber, Jon P
• Assistant Examiner(s): Srivastava, Kailash C
• Application Number:
  US12/224,913
• Publication Number:
  US 20100291610A1
• Time in Patent Office: 2,392 Days
• Field of Search: 435/29, 435/366, 435/377
• US Class Current: 435/366
• CPC Class Codes: A61K 2035/124 the cells being hematopoietic, bone marrow derived or blood cells
• CPC Class Codes: A61K 35/44 Vessels; Vascular smooth muscle cells; Endothelial cells; Endothelial progenitor cells
• CPC Class Codes: C12N 2501/14 Erythropoietin [EPO]
• CPC Class Codes: C12N 2501/165 Vascular endothelial growth factor [VEGF]
• CPC Class Codes: C12N 2501/23 Interleukins [IL]
• CPC Class Codes: C12N 2501/33 Insulin together with transferrin C12N2500/25; Insulin-like growth factors C12N2501/105
• CPC Class Codes: C12N 2501/39 Steroid hormones
• CPC Class Codes: C12N 2501/392 Sexual steroids
• CPC Class Codes: C12N 2501/599 with CD designations not provided for elsewhere
• CPC Class Codes: C12N 2501/91 Heparin
• CPC Class Codes: C12N 2506/11 from blood or immune system cells
• CPC Class Codes: C12N 2510/00 Genetically modified cells
• CPC Class Codes: C12N 2533/52 Fibronectin; Laminin
• CPC Class Codes: C12N 2533/54 Collagen; Gelatin
• CPC Class Codes: C12N 5/0607 Non-embryonic pluripotent stem cells, e.g. MASC induced pluripotent stem cells C12N5/0696
• CPC Class Codes: C12N 5/0634 Cells from the blood or the immune system
• CPC Class Codes: C12N 5/0692 Stem cells; Progenitor cells; Precursor cells

Abstract

A population of cultured cells is provided that comprises at least 10% of a sub-population of cells that: stain as CD31 Bright, demonstrate uptake of Ac-LDL+, and secrete both IL-8 and angiogenin.

Claims

1. A composition of matter, comprising a population of cultured cells that comprises a sub-population of cells that stain as CD31Bright, demonstrate uptake of Ac-LDL+ and secrete interleukin-8 and angiogenin, wherein the sub-population of cells comprises at least 10% of the cells in the population of cultured cells.

2. The composition of matter according to claim 1, wherein the sub-population secretes at least 50 pg interleukin-8 per 10⁶ cells/ml over a period of at least 24 hours.

3. The composition of matter according to claim 1, wherein at least 1.5% of the cells of the population comprise a feature selected from the group consisting of:

CD34, CD117, CD133, Tie-2, CD34+CD133+, KDR, CD34+KDR+, CD144, von Willebrand Factor, SH2 (CD105), SH3, fibronectin, collagen type I, collagen type III, collagen type IV, ICAM type 1, ICAM type 2, VCAM1, vimentin, BMP-R IA, BMP-RII, CD44, integrin b1, aSM-actin, MUC18, CXCR4 and CXCR8.

4. The composition of matter according to claim 1, wherein at least 1.5% of the cells of the population secrete a molecule selected from the group consisting of:

vascular endothelial growth factor, matrix metalloproteinase-2, and matrix metalloproteinase-9.

5. The composition of matter according to claim 1, wherein at least 1.5% of the cells of the population include a feature selected from the group consisting of:

a tube-like structure, a tendency to form a colony, a tendency to form a cluster, and a tendency to migrate toward a chemoattractant.

6. The composition of matter according to claim 1, wherein the sub-population comprises at least 25% of the cells in the population.

7. The composition of matter according to claim 1, wherein the sub-population comprises at least 50% of the cells in the population.

8. The composition of matter according to claim 1, wherein the sub-population secretes at least 150 pg IL-8 per 10⁶ cells/ml over a period of at least 24 hours.

9. The composition of matter according to claim 1, wherein the sub-population secretes at least 1000 pg IL-8 per 10⁶ cells/ml over a period of at least 24 hours.

10. The composition of matter according to claim 1, wherein at least 1.5% of the cells of the population include a morphological feature selected from the group consisting of:

a cell size larger than 20 μ

m, an elongated cell, a spindle-shaped cell, an irregularly-shaped cell, a granulated cell, a cell including an enlarged dark nucleus, a multinuclear cell, a cell including flagella-like structures, a cell including pseudopodia, and a cell having a polygonal shape.

11. The composition of matter according to claim 1, wherein at least 1.5% of the cells of the population have a tendency to migrate toward a chemoattractant selected from the group consisting of:

bFGF, VEGF, SCF, G-CSF, GM-CSF, and SDF-1.

12. The composition of matter according to claim 1, wherein at least 1.5% of the cells of the population have a tendency to migrate toward IL-8.