Implantable biosensor and methods of use thereof

• US 8,543,182 B2
• Filed: 06/29/2006
• Issued: 09/24/2013
• Est. Priority Date: 12/18/2003
• Status: Active Grant

• Current Assignee: Metronom Health Inc.
• Original Assignee: Metronom Health Inc.
• Inventors: Botvinick, Elliot, Bremer, Troy M.
• Primary Examiner(s): TSAY, MARSHA M
• Application Number:
  US11/427,641
• Publication Number:
  US 20060241365A1
• Time in Patent Office: 2,644 Days
• Field of Search: None
• US Class Current: 600/345
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: A61B 5/1455 using optical sensors, e.g. spectral photometrical oximeters
• CPC Class Codes: A61B 5/1459 invasive, e.g. introduced into the body by a catheter A61B5/1464 takes precedence
• CPC Class Codes: A61B 5/1473 invasive, e.g. introduced into the body by a catheter A61B5/1482 takes precedence
• CPC Class Codes: A61B 5/14865 invasive, e.g. introduced into the body by a catheter or needle or using implanted sensors
• CPC Class Codes: A61B 5/1495 Calibrating or testing of in-vivo probes

Abstract

Provided herein is a stabilized oxygen transport matrix that includes a reversible oxygen binding protein, such as hemoglobin, immobilized throughout the stabilized oxygen transport matrix. The stabilized oxygen transport matrix is used to transport oxygen and can be used as an oxygen transport region and a reaction region of an analyte sensor, such as an implantable glucose sensor. The reversible binding protein can also function as an oxygen probe within the analyte sensor.

Claims

1. A device, comprising:

a) a first oxygen permeable surface;

b) a second oxygen permeable surface; and

c) a stabilized oxygen transport matrix in between the first and second oxygen permeable surfaces, wherein the stabilized oxygen transport matrix comprises a reversible oxygen binding protein immobilized throughout the stabilized oxygen transport matrix, and wherein the stabilized oxygen transport matrix transports oxygen from the first oxygen permeable surface to the second oxygen permeable surface.

2. The device of claim 1, wherein the reversible oxygen binding protein is an engineered hemeprotein, or a heme derivative.

3. The device of claim 1, wherein the reversible oxygen binding protein is hemoglobin.

4. The device of claim 1, wherein the reversible oxygen binding protein is present at about 10% by mass or higher.

5. The device of claim 1, further comprising a carrier protein.

6. The device of claim 5, wherein the carrier protein is albumin, gelatin or a combination thereof.

7. The device of claim 5, wherein the carrier protein is present at about 1 to 15% by weight.

8. The device of claim 1, further comprising a polymer selected from the group consisting of polyglycolic acid, polylactic acid, poly (lactic-co-glycolic acid), poly(caprolactone), poly(dioxanone), polytetrafluoroethylene, expanded polytetrafluoroethylene, polypropylene, polyethylene glycol, and polyvinyl alcohol.

9. The device of claim 1, further comprising a coating of silicon, rubber or polymer.

10. The device of claim 1, further comprising a membrane.

11. The device of claim 10, wherein the membrane is a non-natural membrane.

12. The device of claim 10, wherein the membrane is made from a naturally derived material selected from the group consisting of collagen, alginate, agarose, hyaluronic acid derivatives, chitosan, and fibrin glue.

13. The device of claim 10, wherein the membrane is made from a synthetic polymer selected from the group consisting of polyglycolic acid, polylactic acid, poly(lactic-co-glycolic acid), poly(caprolactone), poly(dioxanone), polytetrafluoroethylene, expanded polytetrafluoroethylene, polypropylene, polyethylene glycol, and polyvinyl alcohol.

14. A biosensor, comprising the device of claim 1.

15. The device of claim 1, wherein the partial pressure of oxygen at the first oxygen permeable surface is greater than the partial pressure of oxygen at the second oxygen permeable surface.