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Compound screening using chondrocytes derived from primate pluripotent stem cells

  • US 8,546,101 B2
  • Filed: 02/04/2011
  • Issued: 10/01/2013
  • Est. Priority Date: 12/07/2001
  • Status: Expired due to Fees
First Claim
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1. A method of screening a compound for an effect on cultured chondrocytes, comprising:

  • a) providing primate pluripotent stem cells which express SSEA3, SSEA4, Tra-1-60 and Tra-1-81;

    b) culturing the primate pluripotent stem cells which express SSEA3, SSEA4, Tra-1-60 and Tra-1-81 in a medium comprising one or more of transforming growth factor (TGF), fibroblast growth factor (FGF), growth differentiation factor (GDF), bone morphogenic protein (BMP), hedgehog protein, L-ascorbic acid and para-thyroid hormone related protein to produce cultured chondrocytes;

    c) contacting the cultured chondrocytes with the compound; and

    d) observing a change in the cultured chondrocytes.

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