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In vivo genome-wide mutagenesis

  • US 8,546,135 B2
  • Filed: 02/08/2008
  • Issued: 10/01/2013
  • Est. Priority Date: 02/09/2007
  • Status: Expired due to Fees
First Claim
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1. A gene-trap vector, comprising the formula:


  • 3′

    TR-lox-SA-R1

    X—

    R2-lox-X-5′

    TRwherein 3′

    TR and 5′

    TR are piggyBac 3′ and

    5′

    transposon terminal sequences comprising 3′ and

    5′

    inverted terminal repeats (ITR), respectively;

    wherein the lox sites are in the same orientation;

    wherein SA is a splice acceptor;

    wherein R1 is a first reporter sequence linked to SA; and

    wherein R2 is a second reporter sequence functionally linked to an expression control sequence, and wherein X is a recombination site, wherein X is FRT, attB or attP.

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