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Compensator for multiple surface imaging

  • US 8,546,772 B2
  • Filed: 09/28/2012
  • Issued: 10/01/2013
  • Est. Priority Date: 05/05/2008
  • Status: Active Grant
First Claim
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1. A method for detecting features of an array, comprising:

  • (a) providing a flow cell comprising a first interior surface and a second interior surface that are in a generally mutually facing relation, wherein the first interior surface and the second interior surface are separated by a fluid passage, wherein an array of nucleic acid sites is present on the first interior surface and on the second interior surface, wherein the nucleic acid sites are present at a density of at least 1,000 sites per square millimeter; and

    (b) performing a sequencing by ligation reaction, comprising;

    (i) introducing fluorescent oligonucleotides to the flow cell to add fluorescent tags to the nucleic acid sites;

    (ii) detecting the fluorescent tags at the nucleic acid sites of the array on the first interior surface at a resolution to distinguish the nucleic acid sites on the first interior surface; and

    (iii) repeating steps (b)(i) and (b)(ii).

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