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Amperometric biosensor for histamine determination

  • US 8,551,309 B2
  • Filed: 03/19/2008
  • Issued: 10/08/2013
  • Est. Priority Date: 03/21/2007
  • Status: Expired due to Fees
First Claim
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1. An amperometric biosensor for histamine determination comprising:

  • a substrate;

    a screen-printed working electrode screen-printed onto a surface of the substrate,a counter electrode disposed on the surface of the substrate;

    a reference electrode disposed on the surface of the substrate; and

    a coating of diamine oxidase immobilized with poly (2-hydroxyethyl methacrylate) (photoHEMA) disposed on a surface of the screen-printed working electrode,wherein the coating of immobilized diamine oxidase comprises a ratio of diamine oxidase to photoHEMA of 1;

    4.

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