Amperometric biosensor for histamine determination
First Claim
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1. An amperometric biosensor for histamine determination comprising:
- a substrate;
a screen-printed working electrode screen-printed onto a surface of the substrate,a counter electrode disposed on the surface of the substrate;
a reference electrode disposed on the surface of the substrate; and
a coating of diamine oxidase immobilized with poly (2-hydroxyethyl methacrylate) (photoHEMA) disposed on a surface of the screen-printed working electrode,wherein the coating of immobilized diamine oxidase comprises a ratio of diamine oxidase to photoHEMA of 1;
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Abstract
There is provided by this invention a simple and rapid amperometric biosensor for determining the level of histamine in seafood or fish. The biosensor combines the technology of screen-printing with immobilized diamine oxidase as the bioreceptor. IQ one embodiment of the present invention, the biosensor incorporates potassium hexacyanoferrate (III) as a mediator.
3 Citations
31 Claims
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1. An amperometric biosensor for histamine determination comprising:
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a substrate; a screen-printed working electrode screen-printed onto a surface of the substrate, a counter electrode disposed on the surface of the substrate; a reference electrode disposed on the surface of the substrate; and a coating of diamine oxidase immobilized with poly (2-hydroxyethyl methacrylate) (photoHEMA) disposed on a surface of the screen-printed working electrode, wherein the coating of immobilized diamine oxidase comprises a ratio of diamine oxidase to photoHEMA of 1;
4. - View Dependent Claims (2, 3, 4, 8, 9, 10, 11, 12, 13, 14)
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5. A method of immobilizing diamine oxidase on a surface of a screen-printed working electrode, the method comprising:
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a) mixing a diamine oxidase solution with poly (2-hydroxyethyl methacrylate) (photoHEMA) with a ratio of diamine oxidase to photoHEMA of 1;
4;b) drop-coating the mixture onto a surface of the screen-printed working electrode; and c) photo-curing the screen-printed working electrode. - View Dependent Claims (6, 7)
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15. An amperometric biosensor for histamine determination comprising:
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a substrate; a working electrode screen-printed onto a surface of the substrate; a counter electrode screen-printed onto the surface of the substrate; a reference electrode screen-printed onto the surface of the substrate; a coating of diamine oxidase immobilized with poly (2-hydroxyethyl methacrylate) (photoHEMA) on a surface of the screen-printed working electrode; and potassium hexacyanoferrate (III) electrodeposited on the surface of the screen-printed working electrode, wherein the coating of immobilized diamine oxidase comprises a ratio of diamine oxidase to photoHEMA of 1;
4. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31)
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Specification