Apparatus for processing a sample comprising a biochip and reagents embedded in a biodegradable material, and processes thereof

• US 8,551,424 B2
• Filed: 11/09/2006
• Issued: 10/08/2013
• Est. Priority Date: 11/17/2005
• Status: Active Grant

• Current Assignee: Boehringer Ingelheim Vetmedica GmbH (C.H. Boehringer Sohn AG & Co. KG)
• Original Assignee: Siemens AG
• Inventors: Abraham-Fuchs, Klaus, Ehben, Thomas, Gumbrecht, Walter, Schmidt, Sebastian, Zilch, Christian
• Primary Examiner(s): Gakh, Yelena G
• Application Number:
  US11/594,938
• Publication Number:
  US 20080182338A1
• Time in Patent Office: 2,525 Days
• Field of Search: None
• US Class Current: 422/528
• CPC Class Codes: G01N 1/42 Low-temperature sample treatment, e.g. cryofixation
• CPC Class Codes: G01N 2035/00148 Test cards, e.g. Biomerieux or McDonnel multiwell test cards
• CPC Class Codes: Y10T 436/25 including sample preparation

Abstract

An apparatus is disclosed for processing a sample which stores processing reagents required for processing in a dry form. The processing reagents are covered by a biologically degradable medium or embedded in said biologically degradable medium.

Claims

1. An apparatus for processing a sample, comprising:

a cartridge having at least one microfluidic channel formed therein;

a biochip; and

processing reagents in dry form stored in the cartridge, wherein the processing reagents are embedded in a biologically degradable medium which is not soluble in its non-degraded state in a solvent in which the reagents are dissolved.

2. The apparatus as claimed in claim 1, wherein the processing reagents and the biologically degradable medium form a nonhomogeneous mixture.

3. The apparatus as claimed in claim 1, wherein the processing reagents are present as inclusions inside the degradable medium.

4. The apparatus as claimed in claim 1, wherein the medium comprises at least one biopolymer layer covering an outer surface of the processing reagents.

5. The apparatus as claimed in claim 4, wherein the biopolymer is a protein.

6. The apparatus as claimed in claim 5, wherein the protein is gelatin.

7. The apparatus as claimed in claim 4, wherein the biopolymer is a polysaccharide.

8. The apparatus as claimed in claim 7, wherein the polysaccharide is a dextran.

9. The apparatus as claimed in claim 7, wherein the polysaccharide is starch.

10. The apparatus as claimed in claim 7, wherein the polysaccharide is cellulose.

11. The apparatus as claimed in claim 1, further comprising means for degrading the medium.

12. The apparatus as claimed in claim 11, wherein the means for degrading the medium include at least one enzyme.

13. The apparatus as claimed in claim 11, further comprising:

means for controlling the temperature of the means for degrading the medium, said means for degrading including a temperature-dependent activity.

14. The apparatus as claimed in claim 13, wherein the means for degrading the medium are chosen so as for the medium to be degraded only above a defined temperature.

15. The apparatus as claimed in claim 11, wherein the means for degrading the medium comprise HtrA2.

16. The apparatus as claimed in claim 11, wherein the means for degrading the medium comprise Pyrococcus furiosus alpha-amylase.

17. The apparatus as claimed in claim 11, wherein the means for degrading the medium have a pH-dependent activity.

18. The apparatus as claimed in claim 11, wherein the means for degrading the medium have an ion-dependent activity.

19. The apparatus as claimed in claim 11, wherein the means for degrading the medium are designed as at least one degrading reagent stored in a dry form.

20. The apparatus as claimed in claim 19, wherein the medium is at least partially covered by the degrading reagent.

21. The apparatus as claimed in claim 19, wherein the degrading reagent is at least partially embedded in the medium.

22. The apparatus as claimed in claim 19, wherein the degrading reagent is activatable by contacting the medium with water.

23. The apparatus as claimed in claim 19, wherein the degrading reagent is water-soluble.

24. The apparatus as claimed in claim 1, further comprising:

at least one microchannel or one microcavity for receiving the processing reagents, into which said processing reagents and the degrading reagent have been introduced as mixtures which form stable substances at room temperature.

25. The apparatus as claimed in claim 24, wherein the mixtures are anhydrous solids.

26. The apparatus as claimed in claim 24, wherein at least one of the solids adheres to walls of the microchannel or of the microcavity.

27. An apparatus for processing a sample, comprising:

a cartridge having at least one microfluidic channel formed therein;

a biochip; and

processing reagents in dry form stored in the cartridge, wherein the processing reagents covered by a biologically degradable medium which is not soluble in its non-degraded state in solvent in which the reagents are dissolved.

28. The apparatus as claimed in claim 27, wherein the medium forms a protective layer for the processing reagents.

29. The apparatus as claimed in claim 27, wherein the medium is water-resistant in its non-degraded state.

30. The apparatus as claimed in claim 27, wherein the medium comprises at least one biopolymer.

31. The apparatus as claimed in claim 27, further comprising means for degrading the medium fixed to the at least one microchannel and one microcavity.

32. The apparatus as claimed in claim 31, wherein the means for degrading the medium include at least one enzyme.

33. The apparatus as claimed in claim 31, further comprising:

means for controlling the temperature of the means for degrading the medium, said means for degrading including a temperature-dependent activity.

34. The apparatus as claimed in claim 27, further comprising:

at least one microchannel or one microcavity for receiving the processing reagents, into which said processing reagents and the degrading reagent have been introduced as mixtures which form stable substances at room temperature.

35. A process for processing a sample, comprising:

providing an apparatus for processing a sample, including processing reagents required for processing the sample, stored in a dry form, and a biologically degradable medium that is not soluble in a non-degraded state in a solvent in which the reagents are dissolved, the processing reagents being at least one of covered by and embedded in the biologically degradable medium;

delivering the sample in an aqueous solvent;

degrading the medium by using degrading reagents;

dissolving the processing reagents in the aqueous solvent; and

processing the sample by using the processing reagents.

36. The process as claimed in claim 35, wherein the apparatus contains a reservoir of the processing reagent.

37. The process as claimed in claim 35, wherein the degrading reagent is delivered with the sample.

38. The process as claimed in claim 35, wherein the temperature of the degrading reagent is increased so as for the medium to be degraded.

39. A process for preparing an apparatus for processing a sample, including processing reagents required for processing the sample, stored in a dry form, and a biologically degradable medium that is not soluble in a non-degraded state in a solvent in which the reagents are dissolved, the processing reagents being at least one of covered by and embedded in the biologically degradable medium, the process comprising:

providing a mixture including at least the processing reagents;

at least one of covering and embedding the reagents in the biologically degradable medium;

providing a reaction vessel;

applying the mixture to at least one wall of the reaction vessel;

drying the mixture; and

providing a degrading reagent layer on at least one of the biologically degradable medium and on the wall of the reaction vessel.

40. The process as claimed in claim 39, further comprising applying the mixture to the wall by spotting.

41. A process for preparing an apparatus for processing a sample, including processing reagents required for processing the sample, stored in a dry form, and a biologically degradable medium, water-resistant in a non-degraded state, the processing reagents being at least one of covered by and embedded in the biologically degradable medium, the process comprising:

providing a reaction vessel;

applying the processing reagents to at least one wall of the reaction vessel;

drying the processing reagents;

providing the biologically degradable medium in soluble form;

solublizing the medium using in a solvent that does not dissolve the processing reagents;

applying the dissolved medium to the processing reagents;

drying the dissolved medium; and

covering the biologically degradable medium with a degrading reagent layer.

42. The process as claimed in claim 41, wherein the solvent is ether.

43. The process as claimed in claim 42, wherein drying is carried out by way of freeze-drying.

44. An apparatus for processing a sample, comprising:

processing reagents required for processing the sample, stored in a dry form in the apparatus; and

a biologically degradable medium that is not soluble in a non-degraded state in a solvent in which the reagents are dissolved, the processing reagents being at least one of covered by and embedded in the biologically degradable medium.

45. The apparatus as claimed in claim 44, wherein the processing reagents and the biologically degradable medium form a nonhomogeneous mixture.

46. The apparatus as claimed in claim 44, wherein the processing reagents are present as inclusions inside the degradable medium.

47. The apparatus as claimed in claim 44, further comprising means for degrading the medium covering the degradable medium.

48. The apparatus as claimed in claim 47, wherein the means for degrading the medium include at least one enzyme.

49. The apparatus as claimed in claim 47, further comprising:

means for controlling the temperature of the means for degrading the medium, said means for degrading including a temperature-dependent activity.

50. The apparatus as claimed in claim 44, further comprising:

at least one microchannel and one microcavity for receiving the processing reagents, into which said processing reagents and the degrading reagent have been introduced as mixtures which form stable substances at room temperature.