Method of loading sample into a microfluidic device
First Claim
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1. A method comprising:
- loading a sample into a fluidic device comprising a plurality of sample chambers, said sample chambers having respective volumes ranging from about 1 picoliter to about 1 microliter;
in the fluidic device, subdividing the sample into a plurality of sample portions such that respective sample portions are positioned in the respective sample chambers, such that at least one of said plurality of sample chambers contains a sample portion having a single molecule of a target nucleic acid wherein a detectable concentration of said target nucleic acid will be attained in said at least one sample chamber after a single round of amplification; and
simultaneously subjecting the sample portions loaded into said respective sample chambers to amplification conditions using at least one amplification targeting reagent positioned in said sample chambers.
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Abstract
A method comprising loading a sample into a microfluidic device which comprises plural sample chambers, subdividing the sample into a plurality of sample portions, such that respective sample portions are positioned in each of a plurality of the sample chambers, and subjecting the sample portions loaded into the respective sample chambers to at least a first amplification step. Each of the sample chambers has a respective volume such that if a sample portion positioned in the sample chamber comprises at least one molecule of a target nucleic acid, the target nucleic acid would attain a detectable concentration in the sample chamber after a single round of amplification.
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41 Claims
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1. A method comprising:
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loading a sample into a fluidic device comprising a plurality of sample chambers, said sample chambers having respective volumes ranging from about 1 picoliter to about 1 microliter; in the fluidic device, subdividing the sample into a plurality of sample portions such that respective sample portions are positioned in the respective sample chambers, such that at least one of said plurality of sample chambers contains a sample portion having a single molecule of a target nucleic acid wherein a detectable concentration of said target nucleic acid will be attained in said at least one sample chamber after a single round of amplification; and simultaneously subjecting the sample portions loaded into said respective sample chambers to amplification conditions using at least one amplification targeting reagent positioned in said sample chambers. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41)
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Specification