PCA3, PCA3 genes, and methods of use
First Claim
1. A method of determining whether a sample contains a PCA3 nucleic acid, comprising:
- a) contacting said sample with a nucleic acid molecule 10 to 100 nucleotides in length comprising a sequence of at least 10 consecutive nucleotides contained within SEQ ID NO;
1, SEQ ID NO;
3, SEQ ID NO;
4, SEQ ID NO;
6 or a sequence fully complementary thereto; and
b) detecting binding of said nucleic acid molecule to the PCA3 nucleic acid, whereby it is determined that said sample contains the PCA3 nucleic acid.
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Accused Products
Abstract
The present invention relates, in general, to a prostate-specific antigen, PCA3. In particular, the present invention relates to nucleic acid molecules coding for the PCA3 protein; purified PCA3 proteins and polypeptides; recombinant nucleic acid molecules; cells containing the recombinant nucleic acid molecules; antibodies having binding affinity specifically to PCA3 proteins and polypeptides; hybridomas containing the antibodies; nucleic acid probes for the detection of nucleic acids encoding PCA3 proteins; a method of detecting nucleic acids encoding PCA3 proteins or polypeptides in a sample; kits containing nucleic acid probes or antibodies; bioassays using the nucleic acid sequence, protein or antibodies of this invention to diagnose, assess, or prognose a mammal afflicted with prostate cancer; therapeutic uses; and methods of preventing prostate cancer in an animal.
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Citations
32 Claims
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1. A method of determining whether a sample contains a PCA3 nucleic acid, comprising:
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a) contacting said sample with a nucleic acid molecule 10 to 100 nucleotides in length comprising a sequence of at least 10 consecutive nucleotides contained within SEQ ID NO;
1, SEQ ID NO;
3, SEQ ID NO;
4, SEQ ID NO;
6 or a sequence fully complementary thereto; andb) detecting binding of said nucleic acid molecule to the PCA3 nucleic acid, whereby it is determined that said sample contains the PCA3 nucleic acid. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 31, 32)
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16. A method of determining whether a sample contains a PCA3 nucleic acid, comprising:
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a) contacting said sample with a nucleic acid molecule 10 to 100 nucleotides in length comprising a sequence of at least 10 consecutive nucleotides contained within SEQ ID NO;
1, SEQ ID NO;
3, SEQ ID NO;
4, SEQ ID NO;
6 or a sequence fully complementary thereto,b) detecting binding of said nucleic acid molecule to the PCA3 nucleic acid, whereby it is determined that said sample contains the PCA3 nucleic acid, wherein said nucleic acid molecule does not specifically hybridize to nucleotides 511-985 of SEQ ID NO;
1, nucleotides 346-820 of SEQ ID NO;
3, nucleotides 346-820 of SEQ ID NO;
4 or nucleotides 533-1007 of SEQ ID NO;
6 under stringent conditions of 0.2×
SSC and 0.1°
SDS at 68 degrees C. - View Dependent Claims (17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30)
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Specification