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Controllable strand scission of mini circle DNA

  • US 8,551,704 B2
  • Filed: 02/11/2008
  • Issued: 10/08/2013
  • Est. Priority Date: 02/16/2007
  • Status: Active Grant
First Claim
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1. A method for the synthesis of a nucleic acid comprising:

  • providing a complex, wherein said complex comprises a polymerase, a first template, nucleoside triphosphates and/or analogues thereof, and a primer, wherein said first template comprises at least one selectively cleavable linkage within a nucleotide sequence of the template, and further wherein said first template is a circular template;

    cleaving said selectively cleavable linkage to provide a new terminus in the template in the complex; and

    synthesizing said nucleic acid by polymerase mediated, first template dependent extension of said primer.

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