Use of haploid genomes for genetic diagnosis, modification and multiplication
First Claim
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1. A method for selecting an animal cell comprising a haploid genome, comprising the following steps:
- (i) producing an animal embryo comprising a haploid genome of male or female origin;
(ii) culturing said embryo to produce a multi-celled embryo comprising a haploid genome of male or female origin;
(iii) isolating one or more cells from said multi-celled embryo and screening said one or more cells for a desired genetic makeup; and
(iv) selecting one or more cells determined to comprise the desired genetic makeup.
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Abstract
Methods for propagating haploid genomes of male or female origina and genetic screening and modification thereof are provided. These haploid genomes may be used to produce haploid embryos, and embryonic stem-like cells and differentiated cells. Also, these haploid genomes and cells containing, may be used as nuclear transfer donors to produce diploid nuclear transfer units. These diploid NT units e.g., human NT units, may be used to obtain pluripotent cells and differentiated cells and tissues.
2 Citations
20 Claims
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1. A method for selecting an animal cell comprising a haploid genome, comprising the following steps:
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(i) producing an animal embryo comprising a haploid genome of male or female origin; (ii) culturing said embryo to produce a multi-celled embryo comprising a haploid genome of male or female origin; (iii) isolating one or more cells from said multi-celled embryo and screening said one or more cells for a desired genetic makeup; and (iv) selecting one or more cells determined to comprise the desired genetic makeup. - View Dependent Claims (18, 19, 20)
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2. A method for producing an animal embryo having a desired genetic makeup, the method comprising:
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(i) producing an animal embryo comprising a haploid genome of male or female origin; (ii) culturing said embryo to produce a multi-celled embryo comprising a haploid genome of male or female origin; (iii) isolating one or more cells from said multi-celled embryo and screening said one or more cells for a desired genetic makeup; (iv) selecting said one or more cells determined to comprise the desired genetic makeup; and (v) transferring the nucleus of said one or more cells selected in step (iv) into an oocyte and activating said oocyte to produce an embryo comprising said desired genetic makeup. - View Dependent Claims (3, 4, 5, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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6. A method for producing an animal embryonic stem cell having a desired genetic makeup, the method comprising:
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(i) producing an animal embryo comprising a haploid genome of male or female origin; (ii) culturing said embryo to produce a multi-celled embryo comprising a haploid genome of male or female origin; (iii) isolating one or more cells from said multi-celled embryo and screening said one or more cells for a desired genetic makeup; (iv) selecting said one or more cells determined to comprise the desired genetic makeup; (v) transferring the nucleus of said one or more cells selected in step (iv) into an oocyte and activating said oocyte to produce an embryo comprising said desired genetic makeup; (vi) culturing said embryo comprising a desired genetic makeup produced in step (v) to a stage where the embryo comprises a discernible inner cell mass; and (vii) isolating said inner cell mass and culturing said inner cell mass, thereby producing embryonic stem cells comprising a haploid genome of male or female origin.
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Specification