Nucleic acid-based tests for RhD typing, gender determination and nucleic acid quantification
First Claim
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1. A method for determining the relative amount of fetal nucleic acid in a maternal plasma sample, comprising:
- (a) amplifying at least 10 polymorphic sites in nucleic acid obtained from a maternal plasma sample, which polymorphic sites comprise single nucleotide polymorphisms with a minor allele frequency of greater than 0.3 and are selected from the group consisting of;
rs7551188, rs11247894, rs6687785, rs4487973, rs4648888, rs12089156, rs2050927, rs12125888, rs12143315, rs213624, rs660279, rs635364, rs9431593, rs11166512, rs4329520, rs454782, rs12136370, rs12759642, rs11184494, rs2454175, rs156988, rs10793675, rs1829309, rs1452628, rs4570430, rs12062414, rs7545381, rs6427673, rs10802761, rs642449, rs4839419, rs9324198, rs1192619, rs4657868, rs6426873, rs438981, rs3128688, rs4987351, rs6692911, rs6684679, rs4320829, rs4658481, rs3768458, rs860954, rs10453878, rs10753912, rs1637944 and rs4839282;
(b) quantifying the relative amount of the alleles at said at least 10 polymorphic sites; and
(c) expressing the relative amounts of fetal and maternal alleles as a ratio, wherein the ratio indicates the relative amount of fetal nucleic acid present in the maternal sample.
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Abstract
The invention in part provides nucleic acid-based assays, which are particularly useful for non-invasive prenatal testing. The invention in part provides compositions and methods for RhD typing, detecting the presence of fetal nucleic in a sample, determining the relative amount of fetal nucleic acid in a sample and determining the sex of a fetus, wherein each of the assays may be performed alone or in combination.
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Citations
2 Claims
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1. A method for determining the relative amount of fetal nucleic acid in a maternal plasma sample, comprising:
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(a) amplifying at least 10 polymorphic sites in nucleic acid obtained from a maternal plasma sample, which polymorphic sites comprise single nucleotide polymorphisms with a minor allele frequency of greater than 0.3 and are selected from the group consisting of;
rs7551188, rs11247894, rs6687785, rs4487973, rs4648888, rs12089156, rs2050927, rs12125888, rs12143315, rs213624, rs660279, rs635364, rs9431593, rs11166512, rs4329520, rs454782, rs12136370, rs12759642, rs11184494, rs2454175, rs156988, rs10793675, rs1829309, rs1452628, rs4570430, rs12062414, rs7545381, rs6427673, rs10802761, rs642449, rs4839419, rs9324198, rs1192619, rs4657868, rs6426873, rs438981, rs3128688, rs4987351, rs6692911, rs6684679, rs4320829, rs4658481, rs3768458, rs860954, rs10453878, rs10753912, rs1637944 and rs4839282;(b) quantifying the relative amount of the alleles at said at least 10 polymorphic sites; and (c) expressing the relative amounts of fetal and maternal alleles as a ratio, wherein the ratio indicates the relative amount of fetal nucleic acid present in the maternal sample. - View Dependent Claims (2)
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Specification