BX11 enzymes having xylosidase activity

• US 8,551,751 B2
• Filed: 09/05/2008
• Issued: 10/08/2013
• Est. Priority Date: 09/07/2007
• Status: Expired due to Fees

• Current Assignee: Danisco USA Incorporated (International Flavors & Fragrances Incorporated)
• Original Assignee: Dyadic International (Usa), Inc.
• Inventors: Emalfarb, Mark A., Gusakov, Alexander Vasilievich, Punt, Peter J., Verdoes, Jan Cornelis, Sinitsyn, Arkady Panteleimonovich, Vlasenko, Elena, Hinz, Sandra Wihelmina Agnes, Gosink, Mark, Jiang, Zhijie, Van der Meij, Jacoba
• Primary Examiner(s): FRONDA, CHRISTIAN L
• Application Number:
  US12/205,694
• Publication Number:
  US 20090099079A1
• Time in Patent Office: 1,859 Days
• Field of Search: None
• US Class Current: 435/189
• CPC Class Codes: A61P 29/00 Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• CPC Class Codes: C12N 9/2402 hydrolysing O- and S- glycosyl compounds (3.2.1)
• CPC Class Codes: C12N 9/2408 acting on alpha -1,4-glucosidic bonds
• CPC Class Codes: C12N 9/2434 acting on beta-1,4-glucosidic bonds
• CPC Class Codes: C12N 9/2471 Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
• CPC Class Codes: C12N 9/248 Xylanases
• CPC Class Codes: C12Y 301/01072 Acetylxylan esterase (3.1.1.72)
• CPC Class Codes: C12Y 302/01023 Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
• CPC Class Codes: C12Y 302/01037 Xylan 1,4-beta-xylosidase (3.2.1.37)
• CPC Class Codes: C12Y 302/01055 Alpha-N-arabinofuranosidase (3.2.1.55)
• CPC Class Codes: C12Y 302/01139 Alpha-glucuronidase (3.2.1.139)
• CPC Class Codes: C12Y 302/01151 Xyloglucan-specific endo-beta-1,4-glucanase (3.2.1.151), i.e. endoxyloglucanase
• CPC Class Codes: C12Y 302/01155 Xyloglucan-specific exo-beta-1,4-glucanase (3.2.1.155), i.e. exoxyloglucanase
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel

Abstract

This invention relates to novel enzymes and novel methods for producing the same. More specifically this invention relates to a variety of fungal enzymes. Nucleic acid molecules encoding such enzymes, compositions, recombinant and genetically modified host cells, and methods of use are described. The invention also relates to a method to convert lignocellulosic biomass to fermentable sugars with enzymes that degrade the lignocellulosic material and novel combinations of enzymes, including those that provide a synergistic release of sugars from plant biomass. The invention also relates to a method to release cellular content by degradation of cell walls. The invention also relates to methods to use the novel enzymes and compositions of such enzymes in a variety of other processes, including washing of clothing, detergent processes, biorefining, deinking and biobleaching of paper and pulp, and treatment of waste streams.

Claims

1. An isolated protein comprising an amino acid sequence encoded by the nucleic acid molecule of SEQ ID NO 214 or SEQ ID NO:

216.

3. An isolated fusion protein comprising the isolated protein of claim 1 fused to a protein comprising an amino acid sequence that is heterologous to the isolated protein of claim 1.

4. A kit for degrading a lignocellulosic material to fermentable sugars comprising at least one isolated protein of claim 1.

5. A detergent comprising at least one isolated protein of claim 1.

6. A composition for the degradation of a lignocellulosic material comprising at least one isolated protein of claim 1.

7. A recombinant enzyme isolated from a genetically modified microorganism comprising components suitable for degrading a lignocellulosic material to fermentable sugars, wherein the organism has been genetically modified to express at least one protein of claim 1.

8. The recombinant enzyme of claim 7, wherein the enzyme has been subjected to a purification step.

9. A crude fermentation product produced by culturing the cells from a genetically modified organism comprising components suitable for degrading a lignocellulosic material to fermentable sugars, wherein the crude fermentation product contains at least one protein of claim 1.

10. A detergent composition, comprising at least one isolated protein of claim 1 and at least one surfactant.

11. A composition for degrading cell walls comprising at least one isolated protein of claim 1.

2. An isolated protein comprising an amino acid sequence encoded by SEQ ID NO 215.