DDR1-mediated cell purification of pancreatic endocrine cells through their progenitors
First Claim
1. A method of identification of pancreatic cells comprising cells selected from the group consisting of ductal/endocrine progenitor cells, endocrine progenitor cells, and early endocrine cells, the method comprising contacting a cell population comprising pancreatic cells with a DDR1 (Discoidin Domain Receptor family, member 1) binding reagent and detecting cells that bind the DDR1 binding reagent.
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Abstract
The invention relates to a method of identifying, obtaining and/or quantifying a culture of pancreatic cells selected from the group consisting of ductal/endocrine progenitor cells, endocrine progenitor cells, early endocrine cells, and fully differentiated endocrine cells. Also contemplated is a method of expanding the numbers of such cells as well as sorting such cells. The invention further relates to a selective cell surface marker, DDR1, that permits the selection of a unique subset of cells with pancreatic endocrine progenitor phenotype. Furthermore, the invention relates to isolated cells selected from such cells and compositions thereof.
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Citations
17 Claims
- 1. A method of identification of pancreatic cells comprising cells selected from the group consisting of ductal/endocrine progenitor cells, endocrine progenitor cells, and early endocrine cells, the method comprising contacting a cell population comprising pancreatic cells with a DDR1 (Discoidin Domain Receptor family, member 1) binding reagent and detecting cells that bind the DDR1 binding reagent.
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2. A method of obtaining a culture of pancreatic cells comprising cells selected from the group consisting of ductal/endocrine progenitor cells, pancreatic endocrine progenitor cells, and early endocrine cells comprising:
- contacting a cell population comprising pancreatic cells with a DDR1 binding reagent and separating the cells that binds bind the DDR1 binding reagent in a fraction of DDR1 positive cells from cells that do not bind the DDR1 binding reagent.
- View Dependent Claims (3, 4, 5, 6, 15, 16, 17)
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7. A method of quantifying DDR1 positive cells in an in vitro cell culture comprising pancreatic cells, comprising the steps of a) contacting the cell culture comprising pancreatic cells with a DDR1 binding reagent;
- and b) determining the quantity of cells that exhibit DDR1 as a cell surface marker (DDR1 positive cells).
- View Dependent Claims (8)
Specification