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Solutions, methods, and processes for deprotection of polynucleotides

  • US 8,552,174 B2
  • Filed: 03/23/2006
  • Issued: 10/08/2013
  • Est. Priority Date: 10/31/2005
  • Status: Expired due to Fees
First Claim
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1. A method of performing post-synthesis deprotection of a synthetic precursor of a nucleic acid, comprising:

  • providing a synthetic precursor of a nucleic acid comprising at least one protecting group selected from the group consisting of;

    a base protecting group, a 2′

    -hydroxyl protecting group, and a combination thereof, anddeprotecting at least one of the protecting groups of said synthetic precursor of a nucleic acid by contacting said precursor with a solution comprising an α

    -effect nucleophile, wherein the solution is at a pH of about 4 to 11, and wherein the α

    -effect nucleophile has a pKa of about 4 to 13.

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