Solutions, methods, and processes for deprotection of polynucleotides
First Claim
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1. A method of performing post-synthesis deprotection of a synthetic precursor of a nucleic acid, comprising:
- providing a synthetic precursor of a nucleic acid comprising at least one protecting group selected from the group consisting of;
a base protecting group, a 2′
-hydroxyl protecting group, and a combination thereof, anddeprotecting at least one of the protecting groups of said synthetic precursor of a nucleic acid by contacting said precursor with a solution comprising an α
-effect nucleophile, wherein the solution is at a pH of about 4 to 11, and wherein the α
-effect nucleophile has a pKa of about 4 to 13.
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Accused Products
Abstract
Methods of deprotecting polynucleotides are disclosed. One aspect of the method of deprotecting polynucleotides, among others, includes: providing a polynucleotide, wherein the polynucleotide includes at least one nucleotide monomer that has at least one protecting group selected from the following: a base having a protecting group, a 2′-hydroxyl protecting group, and a combination thereof, and deprotecting at least one of the protecting groups of the polynucleotide by introducing the polynucleotide to a solution including an α-effect nucleophile.
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Citations
35 Claims
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1. A method of performing post-synthesis deprotection of a synthetic precursor of a nucleic acid, comprising:
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providing a synthetic precursor of a nucleic acid comprising at least one protecting group selected from the group consisting of;
a base protecting group, a 2′
-hydroxyl protecting group, and a combination thereof, anddeprotecting at least one of the protecting groups of said synthetic precursor of a nucleic acid by contacting said precursor with a solution comprising an α
-effect nucleophile, wherein the solution is at a pH of about 4 to 11, and wherein the α
-effect nucleophile has a pKa of about 4 to 13. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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16. A method of performing post-synthesis deprotection of a synthetic precursor of a nucleic acid, comprising:
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providing a synthetic precursor of a nucleic acid comprising at least one protecting group selected from the group consisting of;
an exocyclic amino protecting group, an imino protecting group, a 2′
-hydroxyl protecting group, and combinations thereof; anddeprotecting at least one of the protecting groups of the synthetic precursor of a nucleic acid by contacting said precursor with a solution comprising an α
-effect nucleophile, wherein the solution is at a pH of about 4 to 10, and wherein the α
-effect nucleophile has a pKa of about 4 to 13. - View Dependent Claims (17, 18, 19, 20)
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21. A method of performing post-synthesis deprotection of a synthetic precursor of a nucleic acid, comprising:
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providing a synthetic precursor of a nucleic acid comprising at least one exocyclic amino protecting group and optionally comprising at least one protecting group selected from the following;
an imino protecting group, a 2′
-hydroxyl protecting group, and combinations thereof; anddeprotecting at least one of the exocyclic amino protecting groups of said synthetic precursor of a nucleic acid by contacting said precursor with a solution comprising an α
-effect nucleophile, wherein the solution is at a pH of about 4 to 11, and wherein the α
-effect nucleophile has a pKa of about 4 to 13. - View Dependent Claims (22, 23, 24, 25)
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26. A method of performing post-synthesis deprotection of a synthetic precursor of a nucleic acid, comprising:
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providing a synthetic precursor of a nucleic acid comprising at least one 2′
-hydroxyl protecting group and optionally comprising at least one protecting group selected from the group consisting of;
an exocyclic amino protecting group, an imino protecting group, and combinations thereof; anddeprotecting the 2′
-hydroxyl protecting groups of said synthetic precursor of a nucleic acid by contacting said precursor with a solution comprising an α
-effect nucleophile, wherein the solution is at a pH of about 4 to 10, and wherein the α
-effect nucleophile has a pKa of about 4 to 13. - View Dependent Claims (27, 28, 29, 30)
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31. A method of performing post-synthesis deprotection of a synthetic precursor of a nucleic acid, comprising:
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providing a synthetic precursor of a nucleic acid comprising at least one exocyclic amino protecting group and at least one 2′
-hydroxyl group, and optionally comprising at least one imino protecting group; anddeprotecting the exocyclic amino protecting groups and the 2′
-hydroxyl groups of said synthetic precursor of a nucleic acid by contacting said precursor with a solution comprising an α
-effect nucleophile, wherein the solution is at a pH of about 4 to 10, and wherein the α
-effect nucleophile has a pKa of about 4 to 13. - View Dependent Claims (32, 33, 34, 35)
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Specification
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Current AssigneeAgilent Technologies, Inc., The Board of Regents of the University of Colorado (University of Colorado System)
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Original AssigneeAgilent Technologies, Inc., The Board of Regents of the University of Colorado (University of Colorado System)
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InventorsDellinger, Douglas J., Timar, Zoltan, Sierzchala, Agnieszka, Dellinger, Geraldine, Caruthers, Marvin H., Myerson, Joel
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Primary Examiner(s)Crane, Lawrence E
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Application NumberUS11/387,269Publication NumberTime in Patent Office2,756 DaysField of SearchNoneUS Class Current536/25.31CPC Class CodesC07H 21/04 with deoxyribosyl as saccha...Y02P 20/55 Design of synthesis routes,...
