Carrier, process for producing same, bioreactor, and chip for surface plasmon resonance analysis

• US 8,557,194 B2
• Filed: 07/22/2011
• Issued: 10/15/2013
• Est. Priority Date: 07/13/2007
• Status: Expired due to Fees

• Current Assignee: Fujifilm Corporation (Fujifilm Holdings Corporation)
• Original Assignee: Fujifilm Corporation (Fujifilm Holdings Corporation)
• Inventors: Minami, Koichi, Takeuchi, Yohsuke
• Primary Examiner(s): Mui, Christine T
• Application Number:
  US13/188,637
• Publication Number:
  US 20110274846A1
• Time in Patent Office: 816 Days
• Field of Search: 422/57, 422/400, 422/62
• US Class Current: 422/400
• CPC Class Codes: C07K 1/22 Affinity chromatography or related techniques based upon selective absorption processes
• CPC Class Codes: C07K 2319/21 containing a His-tag
• CPC Class Codes: G01N 33/54353 with ligand attached to the carrier via a chemical coupling agent coatings G01N33/54393
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G01N 33/54386 Analytical elements
• CPC Class Codes: G01N 33/54393 Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding

Abstract

A carrier comprises a base plate, a polymeric film, which has been bound on a surface of the base plate, and a ligand, which has been bound with the polymeric film. The ligand has been bound with the polymeric film at a density falling within the range of 1.0×10¹⁶ pieces/mm³ to 3.3×10¹⁸ pieces/mm³. The carrier is produced with a process, comprising the steps of: causing the polymeric film to bind on the base plate, and causing the ligand to bind with the polymeric film, the step of causing the ligand to bind with the polymeric film being performed in an organic solvent.

Claims

1. A process for producing a carrier comprising a base plate, a polymeric film formed on the base plate, and a chelating agent bound to the polymeric film, said process comprising steps of:

forming the polymeric film that includes a thin hydrophilic film constituted by a hydrophilic polymer, the hydrophilic polymer having a molecular weight within a range from 10000 to 2000000, on the base plate by reacting the hydrophilic polymer with the base plate in the state of a thin film; and

reacting the chelating agent and a base additive with the thin hydrophilic film in an organic solvent in order to cause the chelating agent to bind to the polymeric film,

wherein said chelating agent is one or more selected from the group consisting of a nitrilotriacetic acid compound, iminodiacetic acid compound phenanthroline compound, terpyridine compound, bipyridine compound, triethylenetetramine compound, diethylenetriamine compound, tris(carboxymethyl)ethylenediamine compound, diethylenetriaminepentaacetic acid compound, polypyrazolylboric acid compound, 1,4,7-triazocyclononane compound, and dimethylglyoxime compound.

2. A process for producing a carrier as defined in claim 1, wherein:

the base additive is 1,8-diazabicyclo[5.4.0]undec-7-ene.

3. A process for producing a carrier as defined in claim 1, wherein:

the organic solvent is one of dimethyl sulfoxide and N,N-dimethylformamide.

4. A process for producing a carrier as defined in claim 3, wherein:

the organic solvent is dimethyl sulfoxide.

5. A process for producing a carrier as defined in claim 1, wherein the chelating agent is bound to the polymeric film via activated carboxyl groups of the polymeric film.

6. A process for producing a carrier as defined in claim 1, wherein:

the polymeric film formed by the reaction of the hydrophilic polymer and the base plate further contains a self assembling monolayer,

wherein the thin hydrophilic film constituted by a hydrophilic polymer is formed on the self assembling monolayer, and the chelating agent is bound to the thin hydrophilic polymer film.

7. A process for producing a carrier as defined in claim 1, wherein:

the method for causing the hydrophilic polymer to react with the base plate in the state of a thin film is one selected from the group consisting of an extrusion coating technique, a curtain coating technique, a casting technique, a screen printing technique, a spin coating technique, a spray coating technique, a slide beads coating technique, a slit and spin technique, a slit coating technique, a die coating technique, a dip coating technique, a knife coating technique, a blade coating technique, a flow coating technique, a roll coating technique, a wire bar boating technique, and a transfer printing technique.