Use of cellular extracts for skin rejuvenation

• US 8,557,295 B2
• Filed: 05/05/2011
• Issued: 10/15/2013
• Est. Priority Date: 05/11/2006
• Status: Active Grant

• Current Assignee: Regenics AS
• Original Assignee: Regenics AS
• Inventors: Gammelsaeter, Runhild, Remmereit, Jan
• Primary Examiner(s): BERTOGLIO, VALARIE E
• Application Number:
  US13/101,445
• Publication Number:
  US 20120107412A1
• Time in Patent Office: 894 Days
• Field of Search: None
• US Class Current: 424/523
• CPC Class Codes: A61K 35/60 Fish, e.g. seahorses; Fish eggs
• CPC Class Codes: A61K 8/676 Ascorbic acid, i.e. vitamin C
• CPC Class Codes: A61K 8/982 Reproductive organs; Embryos, Eggs
• CPC Class Codes: A61K 8/987 of species other than mammals or birds
• CPC Class Codes: A61Q 19/00 Preparations for care of the skin
• CPC Class Codes: A61Q 19/02 for chemically bleaching or whitening the skin
• CPC Class Codes: A61Q 19/08 Anti-ageing preparations
• CPC Class Codes: A61Q 7/00 Preparations for affecting hair growth

Abstract

The invention describes methods and agents for improving cosmetic appearance, for promoting, improving or restoring health of cells and tissues, preferably skin, and more preferably, for restoring aged or damaged skin to a healthy appearance. In preferred embodiments, the methods and agents comprise active extracts produced from fish eggs. The invention further provides processes for making active fish egg extracts.

Claims

1. A method of treating a subject comprising:

contacting the skin of said subject with a water soluble, cytoplasmic fraction of salmon eggs in an amount effective to cause one or more effects selected from the group consisting of reduction of fine lines in the skin, normalization of skin color, increasing skin water content and hydration, decreasing or normalizing the amount of sebum in the skin, decreasing production of melanin, increasing collagen protein production, increasing collagen gene expression, increasing adult stem cell proliferation, increasing cellular metabolism of carbohydrates, increasing cellular metabolism of lipids, prevention of apoptosis, increasing angiogenesis, upregulation the cell cycle of cells, increasing angiogenesis, increasing the hair cycle, increasing follicular development, and increasing cell proliferation.

2. The method of claim 1, wherein said normalization of skin color comprises a reduction of skin melanin index.

3. The method of claim 1, wherein said normalization of skin color comprises a reduction of skin erythema index.

4. The method of claim 1, wherein said cellular extract comprises about 100 to 380 mg/ml protein in an aqueous solution;

about 0.1 to 10 mg/ml RNA;

about 0.1 to 5 mg/ml DNA and 0.1-10% lipids w/w;

wherein said composition has an osmolarity of from about 330 to 440 mOsm, a pH of from about 5.0 to 7.7, and density of from about 0.8 to 1.4 g/ml.

5. The method of claim 1, wherein said cellular extract is selected from the group consisting of an extract of an activated fish egg cellular extract and an unactivated fish egg cellular extract.

6. The method of claim 5, wherein said fish egg cellular extract is from a fertilized egg.

7. The method of claim 1, wherein said cellular extract is provided in a cream, gel, emulsion, ointment, spray, powder or lotion.

8. The method of claim 1, wherein said cellular extract is a cytoplasmic extract.

9. The method of claim 1, wherein said wherein said effects are on one or more cell-types associated with the skin.

10. The method of claim 9, wherein said cell type associated with the skin is selected from the group consisting of a keratinocyte, fibroblast, melanocyte, and adipocyte.

11. The method of claim 1, wherein said effect is reduction of fine lines in the skin.

12. The method of claim 1, wherein said effect is normalization of skin color.

13. The method of claim 1, wherein said effect is increasing skin water content and hydration.

14. The method of claim 1, wherein said effect is decreasing or normalizing the amount of sebum in the skin.

15. The method of claim 1, wherein said effect is decreasing production of melanin.

16. The method of claim 1, wherein said effect is increasing collagen protein production.

17. The method of claim 1, wherein said effect is increasing collagen gene expression.

18. The method of claim 1, wherein said effect is increasing adult stem cell proliferation.

19. The method of claim 1, wherein said effect is increasing cellular metabolism of carbohydrates.

20. The method of claim 1, wherein said effect is increasing cellular metabolism of lipids.

21. The method of claim 1, wherein said effect is prevention of apoptosis.

22. The method of claim 1, wherein said effect is increasing angiogenesis.

23. The method of claim 1, wherein said effect is upregulation the cell cycle of cells.

24. The method of claim 1, wherein said effect is increasing the hair cycle.

25. The method of claim 1, wherein said effect is increasing follicular development.

26. The method of claim 1, wherein said effect is increasing cell proliferation.