Microorganism producing O-phosphoserine and method of producing L-cysteine or derivatives thereof from O-phosphoserine using the same
First Claim
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1. A method for producing cysteine or a derivative thereof, comprising:
- 1) culturing a recombinant microorganism in which the activity of endogeneous phosphoserine phosphatase (SerB) is reduced and the activity of phosphoglycerate dehydrogenase (SerA) is enhanced, to produce O-sphosphoserine (OPS) wherein the SerA has one selected from the group consisting of amino acid sequences of SEQ ID NOS;
3 to 7; and
2) reacting the OPS of step
1) with a sulfide in presence of O-phosphoserine sulfhydrylase (OPSS) or a microorganism expressing OPSS, to produce cysteine or a derivative thereof.
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Abstract
The present invention provides methods for the production of cysteine or derivates thereof by culturing a microorganism having reduced activity of endogenous phosphoserine phosphatase and enhanced activity of phosphoglycerate dehydrogenase and/or phosphoserine aminotransferase. The O-phosphoserine produced by such an organism can then be reacted with a sulfide in the presence of a sulfydrylase or a microorganism expressing a sulfhydrylase to produce cysteine or a derivative thereof. Microorganisms having the properties noted above are also provided herein.
18 Citations
17 Claims
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1. A method for producing cysteine or a derivative thereof, comprising:
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1) culturing a recombinant microorganism in which the activity of endogeneous phosphoserine phosphatase (SerB) is reduced and the activity of phosphoglycerate dehydrogenase (SerA) is enhanced, to produce O-sphosphoserine (OPS) wherein the SerA has one selected from the group consisting of amino acid sequences of SEQ ID NOS;
3 to 7; and2) reacting the OPS of step
1) with a sulfide in presence of O-phosphoserine sulfhydrylase (OPSS) or a microorganism expressing OPSS, to produce cysteine or a derivative thereof. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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Specification