Transilluminator base and scanner for imaging fluorescent gels, charging devices and portable electrophoresis systems

• US 8,562,802 B1
• Filed: 08/19/2011
• Issued: 10/22/2013
• Est. Priority Date: 02/13/2006
• Status: Active Grant

• Current Assignee: Life Technologies Israel Ltd. (Thermo Fisher Scientific Incorporated)
• Original Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Inventors: Beaudet, Matthew, Margalit, Ilana, Wainstein, Andres, Turkieltaub, Gustavo, Benarieh, Ronen
• Primary Examiner(s): Ball, J. Christopher
• Application Number:
  US13/213,953
• Time in Patent Office: 795 Days
• Field of Search: 204450-470, 204606-621
• US Class Current: 204/466
• CPC Class Codes: G01N 27/44704 Details; Accessories

Abstract

Cassette electrophoresis systems that allow viewing of molecules during the electrophoresis run are disclosed. Cassette electrophoresis bases that reversibly engage light sources, such as light source bases are disclosed. Also disclosed are visible light transillumination systems for viewing a pattern of fluorescence emitted by fluorophores comprising a cassette housing fluorophore-containing material and a base unit to support the cassette. In some aspects the base unit that includes a power supply also houses a light source having output in the visible wavelength region and a filter placed between the light source and the fluorophores. The system is constructed and arranged such that patterns of fluorescence emitted by the fluorophores are viewable. Also described are charging devices for providing charge to gel electrophoresis systems, portable gel electrophoresis systems and methods of use thereof.

Claims

1. A charging device operable to charge an electrophoresis device comprising:

a receptacle having at least one cavity configured to accommodate an electrophoresis device;

at least one connector operable to provide electrical connectivity to the electrophoresis device, wherein the connector is a clamp;

at least one switch operable to removable engage the connector to form a connection with the electrophoresis device; and

a rechargeable battery in electrical connection with the connector, whereby the electrophoresis device may be charged to enable electrophoresis.

2. The charging device of claim 1, the connector further providing mechanical connectivity to the electrophoresis device.

3. The charring device of claim 1, the connector having at least a first end having a configuration complementary to a receptacle in the electrophoresis device, whereby the first of the connects fits into the receptacle of the electrophoresis device.

4. The charging device of claim 1, wherein the connector is a spring clamp.

5. The charging device of claim 1, having at least two connectors.

6. The charging device of claim 1, having at least two switches.

7. The charging device of claim 1, further comprising a power connection to recharge the rechargeable battery.

8. The charging device of claim 1, wherein the electrophoresis device comprises an automated electrophoresis device;

a transilluminator;

an imaging system or any combinations thereof.

9. The charging device of claim 1, wherein the electrophoresis device uses pre-cast gels.

10. A portable electrophoresis system comprising:

a charging device operable to provide charge to an electrophoresis device to enable electrophoresis, the charging device comprising;

a receptacle having at least one cavity configured to accommodate an electrophoresis device;

at least one connector operable to provide electrical connectivity to the electrophoresis device;

at least one switch operable to removable engage the connector to form a connection with the electrophoresis device;

a rechargeable battery in electrical connection with the connector; and

an electrophoresis device, wherein the electrophoresis device comprises a transilluminator.

11. The portable electrophoresis system of claim 10, the charging device further comprising a power connection to recharge the rechargeable battery.

12. The portable electrophoresis system of claim 10, wherein the electrophoresis device comprises an automated electrophoresis device.

13. The portable electrophoresis system of claim 10, wherein the electrophoresis device comprises an imaging system.

14. The portable electrophoresis system of claim 10, wherein the electrophoresis device uses pre-cast gels.

15. The portable electrophoresis system of claim 10, the connector having at least a first end having a configuration complementary to a receptacle in the electrophoresis device, whereby the first end of the connects fits into the receptacle of the electrophoresis device.

16. A method for performing electrophoresis comprising:

placing an electrophoresis base into a portable docking device the portable docking device having a rechargable battery pack;

loading a gel with a sample that is to be separated by electrophoresis;

forming an electrical connection between electrodes of the electrophoresis base and the portable docking device;

running electrophoresis to separate the components of the sample, wherein the electrophoresis is performed at a location where electrical connectivity is unavailable; and

imaging the resolved components of the sample, wherein the imaging is real-time imaging.

17. A portable electrophoresis system comprising:

a charging device operable to provide charge to an electrophoresis device to enable electrophoresis, the charging device comprising;

a receptacle having at least one cavity configured to accommodate an electrophoresis device;

at least one connector operable to provide electrical connectivity to the electrophoresis device;

at least one switch operable to removable engage the connector to form a connection with the electrophoresis device;

a rechargeable battery in electrical connection with the connector; and

an electrophoresis device, wherein the electrophoresis device comprises an imaging system.

18. A charging device operable to charge an electrophoresis device comprising:

a receptacle having at least one cavity configured to accommodate an electrophoresis device;

at least one connector operable to provide electrical connectivity to the electrophoresis device, wherein the connector is a spring clamp;

at least one switch operable to removable engage the connector to form a connection with the electrophoresis device; and

a rechargeable battery in electrical connection with the connector, whereby the electrophoresis device may be charged to enable electrophoresis.