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Methods of culturing cells in a medium comprising transforming growth factor beta 1 and basic fibroblast growth factor

  • US 8,563,311 B2
  • Filed: 05/08/2012
  • Issued: 10/22/2013
  • Est. Priority Date: 12/16/2002
  • Status: Expired due to Term
First Claim
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1. A method of propagating mammalian pluripotent stem cells in an undifferentiated, pluripotent and proliferative state under culturing conditions devoid of feeder cells, the method comprising culturing the mammalian pluripotent stem cells in a tissue culture medium which comprises transforming growth factor β

  • 1 (TGFβ

    1) and basic fibroblast growth factor (bFGF), to thereby maintain the mammalian pluripotent stem cells in an undifferentiated, pluripotent and proliferative state.

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