Synthetic surfaces for culturing stem cell derived cardiomyocytes
First Claim
Patent Images
1. A method for culturing a pluripotent stem cell-derived cardiomyocyte, comprising:
- depositing a suspension comprising the pluripotent stem cell-derived cardiomyocyte on a polymer material; and
culturing the deposited pluripotent stem cell-derived cardiomyocyte in a cell culture medium, wherein the polymer material comprises(i) a homopolymer selected from tetra(ethylene glycol) diacrylate, glycerol dimethacrylate, triethylene glycol dimethacrylate, 1,4-butanediol dimethacrylate, and poly(ethylene glycol) diacrylate;
or(ii) a copolymer formed from;
tetra(ethylene glycol) diacrylate and 1,4-butanediol dimethacrylate,glycerol dimethacrylate and tetra(ethylene glycol) diacrylate,glycerol dimethacrylate and tri(ethylene glycol) dimethacrylate,glycerol dimethacrylate and 1,4-butanediol dimethacrylate,glycerol dimethacrylate and poly(ethylene glycol) diacrylate,triethylene glycol dimethacrylate and 1,4-butanediol dimethacrylate,poly(ethylene glycol) diacrylate and tetra(ethylene glycol) diacrylate,di(ethylene glycol) dimethacrylate and tetraethylene glycol dimethacrylate,tetra(ethylene glycol) diacrylate and trimethylolpropane triacrylate,trimethylolpropane triacrylate and neopentyl glycol ethoxylate diacrylate, ortetra(ethylene glycol) dimethacrylate and di(ethylene glycol) dimethacrylate.
1 Assignment
0 Petitions
Accused Products
Abstract
Synthetic surfaces suitable for culturing stem cell derived cardiomyocytes contain acrylate polymers formed from one or more acrylate monomers. The acrylate surfaces, in many cases, are suitable for culturing stem cell derived cardiomyocytes in chemically defined media.
-
Citations
17 Claims
-
1. A method for culturing a pluripotent stem cell-derived cardiomyocyte, comprising:
-
depositing a suspension comprising the pluripotent stem cell-derived cardiomyocyte on a polymer material; and culturing the deposited pluripotent stem cell-derived cardiomyocyte in a cell culture medium, wherein the polymer material comprises (i) a homopolymer selected from tetra(ethylene glycol) diacrylate, glycerol dimethacrylate, triethylene glycol dimethacrylate, 1,4-butanediol dimethacrylate, and poly(ethylene glycol) diacrylate;
or(ii) a copolymer formed from; tetra(ethylene glycol) diacrylate and 1,4-butanediol dimethacrylate, glycerol dimethacrylate and tetra(ethylene glycol) diacrylate, glycerol dimethacrylate and tri(ethylene glycol) dimethacrylate, glycerol dimethacrylate and 1,4-butanediol dimethacrylate, glycerol dimethacrylate and poly(ethylene glycol) diacrylate, triethylene glycol dimethacrylate and 1,4-butanediol dimethacrylate, poly(ethylene glycol) diacrylate and tetra(ethylene glycol) diacrylate, di(ethylene glycol) dimethacrylate and tetraethylene glycol dimethacrylate, tetra(ethylene glycol) diacrylate and trimethylolpropane triacrylate, trimethylolpropane triacrylate and neopentyl glycol ethoxylate diacrylate, or tetra(ethylene glycol) dimethacrylate and di(ethylene glycol) dimethacrylate. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
-
Specification