Synthetic surfaces for culturing stem cell derived cardiomyocytes

• US 8,563,312 B2
• Filed: 07/11/2012
• Issued: 10/22/2013
• Est. Priority Date: 01/30/2008
• Status: Active Grant

• Current Assignee: Asterias Biotherapeutics Incorporated (Lineage Cell Therapeutics, Inc.)
• Original Assignee: Geron Corporation
• Inventors: Shogbon, Christopher Bankole, Zhou, Yue, Brandenberger, Ralph
• Primary Examiner(s): BERTOGLIO, VALARIE E
• Application Number:
  US13/546,381
• Publication Number:
  US 20120276626A1
• Time in Patent Office: 468 Days
• Field of Search: 435/395
• US Class Current: 435/395
• CPC Class Codes: C12N 2500/90 Serum-free medium, which may still contain naturally-sourced components
• CPC Class Codes: C12N 2506/02 from embryonic cells
• CPC Class Codes: C12N 2533/30 Synthetic polymers
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06
• CPC Class Codes: C12N 5/0657 Cardiomyocytes; Heart cells

Abstract

Synthetic surfaces suitable for culturing stem cell derived cardiomyocytes contain acrylate polymers formed from one or more acrylate monomers. The acrylate surfaces, in many cases, are suitable for culturing stem cell derived cardiomyocytes in chemically defined media.

Claims

1. A method for culturing a pluripotent stem cell-derived cardiomyocyte, comprising:

depositing a suspension comprising the pluripotent stem cell-derived cardiomyocyte on a polymer material; and

culturing the deposited pluripotent stem cell-derived cardiomyocyte in a cell culture medium, wherein the polymer material comprises

(i) a homopolymer selected from tetra(ethylene glycol) diacrylate, glycerol dimethacrylate, triethylene glycol dimethacrylate, 1,4-butanediol dimethacrylate, and poly(ethylene glycol) diacrylate;

or

(ii) a copolymer formed from;

tetra(ethylene glycol) diacrylate and 1,4-butanediol dimethacrylate,

glycerol dimethacrylate and tetra(ethylene glycol) diacrylate,

glycerol dimethacrylate and tri(ethylene glycol) dimethacrylate,

glycerol dimethacrylate and 1,4-butanediol dimethacrylate,

glycerol dimethacrylate and poly(ethylene glycol) diacrylate,

triethylene glycol dimethacrylate and 1,4-butanediol dimethacrylate,

poly(ethylene glycol) diacrylate and tetra(ethylene glycol) diacrylate,

di(ethylene glycol) dimethacrylate and tetraethylene glycol dimethacrylate,

tetra(ethylene glycol) diacrylate and trimethylolpropane triacrylate,

trimethylolpropane triacrylate and neopentyl glycol ethoxylate diacrylate, or

tetra(ethylene glycol) dimethacrylate and di(ethylene glycol) dimethacrylate.

2. The method of claim 1, wherein the polymer material comprises:

(i) a homopolymer selected from glycerol dimethacrylate, triethylene glycol dimethacrylate, 1,4-butanediol dimethacrylate, and poly(ethylene glycol) diacrylate;

or

(ii) a copolymer formed from;

tetra(ethylene glycol) diacrylate and 1,4-butanediol dimethacrylate,

glycerol dimethacrylate and tetra(ethylene glycol) diacrylate,

glycerol dimethacrylate and tri(ethylene glycol) dimethacrylate,

glycerol dimethacrylate and 1,4-butanediol dimethacrylate,

glycerol dimethacrylate and poly(ethylene glycol) diacrylate,

triethylene glycol dimethacrylate and 1,4-butanediol dimethacrylate,

poly(ethylene glycol) diacrylate and tetra(ethylene glycol) diacrylate, or

trimethylolpropane triacrylate and neopentyl glycol ethoxylate diacrylate.

3. The method of claim 1, wherein the polymer material comprises:

(i) a homopolymer selected from glycerol dimethacrylate, 1,4-butanediol dimethacrylate, and poly(ethylene glycol) diacrylate;

or

(ii) a copolymer formed from;

glycerol dimethacrylate and tetra(ethylene glycol) diacrylate,

poly(ethylene glycol) diacrylate and tetra(ethylene glycol) diacrylate, or

trimethylolpropane triacrylate and neopentyl glycol ethoxylate diacrylate.

4. The method of claim 1, wherein the polymer material comprises glycerol dimethacrylate.

5. The method of claim 1, wherein the polymer material comprises triethylene glycol dimethacrylate.

6. The method of claim 1, wherein the polymer material comprises 1,4-butanediol dimethacrylate.

7. The method of claim 1, wherein the polymer material comprises poly(ethylene glycol) diacrylate.

8. The method of claim 1, wherein the polymer material comprises a copolymer formed from tetra(ethylene glycol) diacrylate and 1,4-butanediol dimethacrylate.

9. The method of claim 1, wherein the polymer material comprises a copolymer formed from glycerol dimethacrylate and tetra(ethylene glycol) diacrylate.

10. The method of claim 1, wherein the polymer material comprises a copolymer formed from glycerol dimethacrylate and tri(ethylene glycol) dimethacrylate.

11. The method of claim 1, wherein the polymer material comprises a copolymer formed from glycerol dimethacrylate and 1,4-butanediol dimethacrylate.

12. The method of claim 1, wherein the polymer material comprises a copolymer formed from glycerol dimethacrylate and poly(ethylene glycol) diacrylate.

13. The method of claim 1, wherein the polymer material comprises a copolymer formed from triethylene glycol dimethacrylate and 1,4-butanediol dimethacrylate.

14. The method of claim 1, wherein the polymer material comprises a copolymer formed from poly(ethylene glycol) diacrylate and tetra(ethylene glycol) diacrylate.

15. The method of claim 1, wherein the polymer material comprises a copolymer formed from trimethylolpropane triacrylate and neopentyl glycol ethoxylate diacrylate.

16. The method of claim 1, wherein culturing the deposited pluripotent stem cell-derived cardiomyocyte in a cell culture medium comprises culturing the cardiomyocyte in a chemically defined medium.

17. The method of claim 1, wherein the pluripotent stem cell-derived cardiomyocyte is a human embryonic stem cell-derived cardiomyocyte.