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Synthetic surfaces for culturing stem cell derived cardiomyocytes

  • US 8,563,312 B2
  • Filed: 07/11/2012
  • Issued: 10/22/2013
  • Est. Priority Date: 01/30/2008
  • Status: Active Grant
First Claim
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1. A method for culturing a pluripotent stem cell-derived cardiomyocyte, comprising:

  • depositing a suspension comprising the pluripotent stem cell-derived cardiomyocyte on a polymer material; and

    culturing the deposited pluripotent stem cell-derived cardiomyocyte in a cell culture medium, wherein the polymer material comprises(i) a homopolymer selected from tetra(ethylene glycol) diacrylate, glycerol dimethacrylate, triethylene glycol dimethacrylate, 1,4-butanediol dimethacrylate, and poly(ethylene glycol) diacrylate;

    or(ii) a copolymer formed from;

    tetra(ethylene glycol) diacrylate and 1,4-butanediol dimethacrylate,glycerol dimethacrylate and tetra(ethylene glycol) diacrylate,glycerol dimethacrylate and tri(ethylene glycol) dimethacrylate,glycerol dimethacrylate and 1,4-butanediol dimethacrylate,glycerol dimethacrylate and poly(ethylene glycol) diacrylate,triethylene glycol dimethacrylate and 1,4-butanediol dimethacrylate,poly(ethylene glycol) diacrylate and tetra(ethylene glycol) diacrylate,di(ethylene glycol) dimethacrylate and tetraethylene glycol dimethacrylate,tetra(ethylene glycol) diacrylate and trimethylolpropane triacrylate,trimethylolpropane triacrylate and neopentyl glycol ethoxylate diacrylate, ortetra(ethylene glycol) dimethacrylate and di(ethylene glycol) dimethacrylate.

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