Fiber-optic biosensor and biosensing methods
First Claim
1. A biosensing method for simultaneously detecting and quantifying two or more target biomarkers, said method comprising:
- providing at least two optical waveguides, each of the at least two optical waveguides being disposed at least partially in a respective microfluidic channel of a serpentine shaped chamber, the at least two optical waveguides being fluidly coupled to one another in series within the serpentine shaped chamber;
selecting 1°
Mabs corresponding to each of the two or more target biomarkers;
immobilizing the 1°
Mabs on the optical waveguides;
exposing to the 1°
Mabs a sample believed to contain the target biomarkers using convective flow within the serpentine shaped chamber, wherein the sample flows through the serpentine shaped chamber and flows over each of the at least two optical waveguides in series;
providing fluorophore-tagged 2°
Mabs corresponding to the target biomarkers;
exciting the at least two optical waveguides; and
observing a fluorescence emission to determine the presence and quantity of the target biomarkers.
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Abstract
A method for biosensing that includes passing, via convective flow, a sample believed to contain one or more target biomarkers through a microfluidic channel and over the surface of an optical waveguide that has been prepared to bind the one or more target biomarkers, and sensing for an emission output from the optical waveguide at a wavelength that is characteristic of the binding of the target biomarker. A biosensor device that includes a module defining at least one microfluidic channel, an optical waveguide exposed along at least a portion of its length to fluid flow within the microfluidic channel, where a surface of the optical waveguide being prepared to bind a target biomarker, and an excitation source to couple an excitation wavelength of light into the optical waveguide. The device also includes a sensor for detecting emission light from the optical waveguide at an emission wavelength characteristic of binding of the target biomarker.
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Citations
18 Claims
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1. A biosensing method for simultaneously detecting and quantifying two or more target biomarkers, said method comprising:
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providing at least two optical waveguides, each of the at least two optical waveguides being disposed at least partially in a respective microfluidic channel of a serpentine shaped chamber, the at least two optical waveguides being fluidly coupled to one another in series within the serpentine shaped chamber; selecting 1°
Mabs corresponding to each of the two or more target biomarkers;immobilizing the 1°
Mabs on the optical waveguides;exposing to the 1°
Mabs a sample believed to contain the target biomarkers using convective flow within the serpentine shaped chamber, wherein the sample flows through the serpentine shaped chamber and flows over each of the at least two optical waveguides in series;providing fluorophore-tagged 2°
Mabs corresponding to the target biomarkers;exciting the at least two optical waveguides; and observing a fluorescence emission to determine the presence and quantity of the target biomarkers. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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Specification