Polymer conjugate enhanced bioassays
First Claim
Patent Images
1. An assay kit for detecting a target in a sample, comprising:
- a) a detector that specifically binds said target of said sample, said detector tagged with a plurality of a first member of a first binding pair;
b) a conjugate comprising a modified dendritic polymer and a second member of said first binding pair;
(c) a capture molecule comprising a member of a second binding pair which specifically binds said target; and
(d) a reporter.
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Abstract
Modified branched polymers are combined with bioactive agents which are one member of a binding pair for use in an assay.
55 Citations
21 Claims
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1. An assay kit for detecting a target in a sample, comprising:
- a) a detector that specifically binds said target of said sample, said detector tagged with a plurality of a first member of a first binding pair;
b) a conjugate comprising a modified dendritic polymer and a second member of said first binding pair;
(c) a capture molecule comprising a member of a second binding pair which specifically binds said target; and
(d) a reporter.
- a) a detector that specifically binds said target of said sample, said detector tagged with a plurality of a first member of a first binding pair;
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2. The kit of claim 1, wherein said first binding pair comprises an antibody, an antigen binding portion thereof, an antigen, an epitope-containing portion thereof, a nucleic acid, a nucleic acid binding moiety, a lectin, a cognate saccharide thereof, a ligand, a hapten, a hormone, a hormone receptor, an enzyme, an enzyme cofactor, an enzyme substrate, a biotin or a molecule that binds biotin.
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3. The kit of claim 1, wherein said reporter comprises a colored, luminescent or fluorescent particulate or moiety, an enzyme, or a combination thereof.
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4. The kit of claim 3, wherein said fluorescent or luminescent particulate or moiety comprises quantum dots, nanocrystals, up-converting phosphorescent particles or fluorophore containing latex beads.
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5. The kit of claim 3, wherein said colored particulate comprises colloidal metals, comprising gold or silver, colored latex beads or colored dyes.
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6. The kit of claim 1, wherein said conjugate (b) and said reporter (c) are joined.
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7. The kit of claim 1, wherein said kit comprises a solid phase comprising said capture molecule, said detector, said conjugate and said reporter.
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8. The kit of claim 7, wherein said solid phase comprises a flat surface or a membrane.
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9. The kit of claim 8, wherein said flat surface comprises a silicon wafer, quartz, glass, a metal, or a plastic.
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10. The kit of claim 8, wherein said membrane comprises a paper, a plastic membrane, a nylon membrane or a nitrocellulose membrane.
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11. The kit of claim 7, wherein said solid phase comprises a microarray or a bead array.
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12. The kit of claim 7, wherein said solid phase comprises a dipstick, a lateral flow immunoassay or a microarray.
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13. The kit of claim 7, wherein said capture molecule is affixed directly or indirectly to said solid phase.
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14. The kit of claim 7, wherein said conjugate is reversibly bound to said solid phase.
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15. The kit of claim 7, wherein said solid phase comprises a microtitre plate.
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16. The kit of claim 1, wherein said reporter yields a product which is detectable by color or by light.
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17. The kit of claim 1, wherein said first member or said second member of said first binding pair is an antibody or antigen-binding portion thereof.
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18. The kit of claim 1, wherein said first member or said second member of said first binding pair is a molecule that binds biotin, fluorescein, nucleic acid, albumin, a hapten or a combination thereof.
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19. The kit of claim 1, wherein said detector is an antibody, an antigen-binding portion thereof, an antigen, an epitope-containing portion thereof, a nucleic acid or a nucleic acid binding moiety.
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20. The kit of claim 1, where said first member or said second member of said first binding pair comprises biotin, fluorescein, nucleic acid, albumin, a hapten or a combination thereof.
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21. The kit of claim 1, further comprising a housing.
Specification