Fluorescent proteins and methods for using same
First Claim
1. An isolated nucleic acid encoding a functional fluorescent protein having an amino acid sequence that differs from the amino acid sequence of wild-type EqFP578 (SEQ ID NO:
- 2) by at least one amino acid substitution selected from the group consisting of R155E, Q159D, S173N, and F192V.
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Accused Products
Abstract
The present invention provides nucleic acid molecules encoding novel red fluorescent proteins from Entacmaea quadricolor and mutants thereof. Also of interest are proteins that are substantially similar to the novel red fluorescent proteins. In addition, host cells, stable cell lines and transgenic organisms comprising the nucleic acid molecules encoding the novel red fluorescent proteins are provided. The subject proteins and nucleic acid compositions find use in a variety of different applications and methods, particularly for labeling of biomolecules, cells, or cell organelles. Finally, kits for use in such methods and applications are provided.
47 Citations
9 Claims
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1. An isolated nucleic acid encoding a functional fluorescent protein having an amino acid sequence that differs from the amino acid sequence of wild-type EqFP578 (SEQ ID NO:
- 2) by at least one amino acid substitution selected from the group consisting of R155E, Q159D, S173N, and F192V.
- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
Specification