Methods and compositions involving miRNA and miRNA inhibitor molecules
First Claim
Patent Images
1. A synthetic RNA molecule comprising two polynucleotides each between 17 and 25 residues in length comprisinga) a first polynucleotide comprising an miRNA region whose sequence from 5′
- to 3′
is identical to a mature miRNA sequence, andb) a second polynucleotide comprising a complementary region whose sequence from 5′
to 3′
is 100% complementary to the miRNA sequence,wherein i) the 5′
terminus of the second polynucleotide is replaced with a lower alkylamine group and/or ii) there are one or more sugar modifications with a 2′
oxygen-methyl (2′
O-Me) in the first or last 1 to 6 residues of the second polynucleotide.
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Abstract
The present invention concerns methods and compositions for introducing miRNA activity or function into cells using synthetic nucleic acid molecules. Moreover, the present invention concerns methods and compositions for identifying miRNAs with specific cellular functions that are relevant to therapeutic, diagnostic, and prognostic applications wherein synthetic miRNAs and/or miRNA inhibitors are used in library screening assays.
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Citations
9 Claims
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1. A synthetic RNA molecule comprising two polynucleotides each between 17 and 25 residues in length comprising
a) a first polynucleotide comprising an miRNA region whose sequence from 5′ - to 3′
is identical to a mature miRNA sequence, andb) a second polynucleotide comprising a complementary region whose sequence from 5′
to 3′
is 100% complementary to the miRNA sequence,wherein i) the 5′
terminus of the second polynucleotide is replaced with a lower alkylamine group and/or ii) there are one or more sugar modifications with a 2′
oxygen-methyl (2′
O-Me) in the first or last 1 to 6 residues of the second polynucleotide. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
- to 3′
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9. A synthetic RNA molecule comprising two polynucleotides each between 17 and 25 residues in length comprising
a) a first polynucleotide comprising an miRNA region whose sequence from 5′ - to 3′
is identical to a mature miRNA sequence, andb) a second polynucleotide comprising a complementary region whose sequence from 5′
to 3′
is 100% complementary to the miRNA sequence,wherein the 5′
terminus of the second polynucleotide is replaced with a lower alkylamine group.
- to 3′
Specification