Biosensor, biosensor chip and biosensor device

• US 8,568,579 B2
• Filed: 11/13/2009
• Issued: 10/29/2013
• Est. Priority Date: 07/02/2002
• Status: Active Grant

• Current Assignee: PHC Holdings Corporation
• Original Assignee: Panasonic Corporation (Panasonic Holdings Corporation)
• Inventors: Ueno, Hiroya, Nakatsuka, Junji
• Primary Examiner(s): Noguerola, Alex
• Application Number:
  US12/618,084
• Publication Number:
  US 20100140087A1
• Time in Patent Office: 1,446 Days
• Field of Search: 20440301-40315, 205/777.5, 205/778, 205/792
• US Class Current: 204/403.02
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: G01N 27/30 Electrodes, e.g. test electrodes; Half-cells G01N27/414 takes precedence
• CPC Class Codes: G01N 27/307 Disposable laminated or multilayered electrodes G01N27/3272 takes precedence
• CPC Class Codes: G01N 27/327 Biochemical electrodes , e.g. electrical or mechanical details for in vitro measurements
• CPC Class Codes: G01N 27/3271 Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood amperometry per se G01N27/49; aspects concerning the enzyme reagent C12Q1/001
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52
• CPC Class Codes: G01N 27/3273 Devices therefor, e.g. test element readers, circuitry details not specific to biochemical electrodes G01N33/4875
• CPC Class Codes: G01N 27/416 Systems G01N27/27 takes precedence
• CPC Class Codes: G01R 19/0092 measuring current only all subgroups of G01R19/00 take precedence

Abstract

A biosensor includes a working electrode 101, a counter electrode 102 opposing the working electrode 101, a working electrode terminal 103 and a working electrode reference terminal 10 connected to the working electrode 101 by wires, and a counter electrode terminal 104 connected to the counter electrode 102 by a wire. By employing a structure with at least three electrodes, it is possible to assay a target substance without being influenced by the line resistance on the working electrode side.

Claims

1. A biosensor device, comprising:

a biosensor, comprising;

a working electrode;

a counter electrode;

a working electrode terminal;

a counter electrode terminal;

a reference terminal;

the working electrode directly connected to the working electrode terminal and to the reference terminal; and

a measurement circuit connected to the working electrode terminal, the counter electrode terminal, and the reference terminal of the biosensor, wherein the measurement circuit uses the voltage at the reference terminal to control a voltage between the working electrode and the counter electrode.

2. The biosensor device of claim 1, wherein the measurement circuit performs an assay of a fluid that is applied to the biosensor to determine the concentration of a substance in the fluid.

3. The biosensor device of claim 2, wherein the measurement circuit controls the voltage between the working electrode and the counter electrode to be constant.

4. The biosensor device of claim 3, wherein the measurement circuit comprises a voltage application section having an input coupled to the counter electrode reference terminal and an output coupled to the counter electrode terminal.

5. The biosensor device of claim 4, wherein:

the voltage application section includes an amplifier; and

the counter electrode terminal is coupled to an output of the amplifier.

6. The biosensor device of claim 5, wherein the amplifier is an operational amplifier.

7. The biosensor device of claim 6, wherein the amplifier has an input coupled to a base voltage.

8. The biosensor device of claim 4, wherein the voltage application section comprises:

a first amplifier having an input coupled to the counter electrode reference terminal and an output; and

a second amplifier having an input coupled to the output of the first amplifier and an output coupled to the counter electrode terminal.

9. The biosensor device of claim 8, wherein the first amplifier is an operational amplifier.

10. The biosensor device of claim 9, wherein the second amplifier is an operational amplifier.

11. The biosensor device of claim 8, wherein the measurement circuit compensates for the effect of the line resistances of the first conductive line and the third conductive line on the voltage between the working electrode and the counter electrode.

12. The biosensor device of claim 4, wherein:

the voltage application section includes an amplifier; and

the counter electrode reference terminal is coupled to an input of the amplifier.

13. The biosensor device of claim 12, wherein the amplifier is an operational amplifier.

14. A biosensor device, comprising:

a biosensor, comprising;

a working electrode;

a counter electrode;

a working electrode terminal;

a counter electrode terminal;

a reference terminal;

the working electrode connected to the working electrode terminal; and

the counter electrode directly connected to the counter electrode terminal and to the reference terminal; and

a measurement circuit connected to the working electrode terminal, the counter electrode terminal, and the reference terminal of the biosensor, wherein the measurement circuit uses the voltage at the reference terminal to control a voltage between the working electrode and the counter electrode.

15. A biosensor device, comprising:

a biosensor, comprising;

a working electrode;

a counter electrode;

a working electrode terminal;

a counter electrode terminal;

a reference terminal;

a first conductive line connecting the working electrode to the working electrode terminal;

a second conductive line connecting the working electrode to the reference terminal; and

a third conductive line connecting the counter electrode to the counter electrode terminal; and

a measurement circuit connected to the working electrode terminal, the counter electrode terminal, and the reference terminal of the biosensor, wherein the measurement circuit uses the voltage at the reference terminal to control a voltage between the working electrode and the counter electrode.

16. The biosensor device of claim 15, wherein the first conductive line and the third conductive line each exhibit a line resistance.

17. The biosensor device of claim 15, wherein substantially no current flows through the second conductive line.

18. A biosensor device, comprising:

a biosensor, comprising;

a working electrode;

a counter electrode;

a working electrode terminal;

a counter electrode terminal;

a reference terminal;

a first conductive line connecting the working electrode to the working electrode terminal;

a second conductive line connecting the counter electrode to the reference terminal; and

a third conductive line connecting the counter electrode to the counter electrode terminal; and

a measurement circuit connected to the working electrode terminal, the counter electrode terminal, and the reference terminal of the biosensor, wherein the measurement circuit uses the voltage at the reference terminal to control a voltage between the working electrode and the counter electrode.

19. A biosensor device, comprising:

a biosensor, comprising;

a working electrode;

a counter electrode;

a working electrode terminal;

a counter electrode terminal;

a first reference terminal;

a second reference terminal;

the working electrode directly connected to the working electrode terminal and to the first reference terminal; and

the counter electrode directly connected to the counter electrode terminal and to the second reference terminal; and

a measurement circuit connected to the working electrode terminal, the counter electrode terminal, the first reference terminal, and the second reference terminal of the biosensor, wherein the measurement circuit uses the voltages at the first reference terminal and the second reference terminal to control a voltage between the working electrode and the counter electrode.

20. A biosensor device, comprising:

a biosensor, comprising;

a working electrode;

a counter electrode;

a working electrode terminal;

a counter electrode terminal;

a first reference terminal;

a second reference terminal;

a first conductive line connecting the working electrode to the working electrode terminal;

a second conductive line connecting the working electrode to the first reference terminal;

a third conductive line connecting the counter electrode to the counter electrode terminal; and

a fourth conductive line connecting the counter electrode and to the second reference terminal; and

a measurement circuit connected to the working electrode terminal, the counter electrode terminal, the first reference terminal, and the second reference terminal of the biosensor, wherein the measurement circuit uses the voltages at the first reference terminal and the second reference terminal to control a voltage between the working electrode and the counter electrode.

21. The biosensor device of claim 20, wherein substantially no current flows through the fourth conductive line.