Microarray with microchannels

• US 8,569,046 B2
• Filed: 02/20/2009
• Issued: 10/29/2013
• Est. Priority Date: 02/20/2009
• Status: Active Grant

• Current Assignee: Massachusetts Institute of Technology
• Original Assignee: Massachusetts Institute of Technology
• Inventors: Love, John Christopher, Papa, Eliseo, Story, Craig M.
• Primary Examiner(s): Bowers, Nathan
• Assistant Examiner(s): Prakash, Gautam
• Application Number:
  US12/390,279
• Publication Number:
  US 20100216228A1
• Time in Patent Office: 1,712 Days
• Field of Search: 506/7, 506/12, 506/13, 506/15, 506/32, 506/33, 216/39, 216/52
• US Class Current: 435/288.4
• CPC Class Codes: B01L 2300/0854 Double walls
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: B01L 9/56 Means for indicating position of a recipient or sample in an array
• CPC Class Codes: C12M 23/12 Well or multiwell plates C12M25/04 takes precedence
• CPC Class Codes: C12M 23/16 Microfluidic devices; Capillary tubes integrated microfluidic structures B01L3/5027; microreactors B01J19/0093

Abstract

A microarray formed in a planar surface of a moldable slab, the microarray including a plurality of microwell sets comprising a plurality of microwells formed in the planar surface of the moldable slab, each microwell being sized to contain at least a single cell, and a plurality of microchannels formed in the planar surface of the moldable slab, the plurality of microchannels being configured to permit liquid from a first region of the microarray to transit to a second region of the microarray.

Claims

1. A microarray formed in a planar surface of a moldable slab, the microarray comprising:

a plurality of microchannels formed in the planar surface of the moldable slab, the plurality of microchannels being configured to form a lattice that defines a plurality of interlineal regions; and

a plurality of microwell sets, each of which is positioned in one of the interlineal regions, wherein each set comprises a plurality of microwells formed in the planar surface of the moldable slab, and each microwell is sized to contain at least a single cell, wherein the microchannels and microwells are arranged and constructed in the planar surface of the moldable slab such that, when the planar surface of the moldable slab is placed in contact with a planar surface of a substrate such that a seal is formed between the moldable slab and the substrate, liquid on the planar surface of the moldable slab is excluded from an interfacial region, via the microchannels.

2. The microarray of claim 1, wherein the microchannels and microwells are arranged and constructed in the planar surface of the moldable slab such that, when the liquid is excluded from the interfacial region via the microchannels, each microwell retains sufficient liquid to sustain at least one cell therein.

3. The microarray of claim 1, wherein the plurality of microchannels comprises a circumferential microchannel in fluid communication with a port such that aspiration of the microchannel can be performed via the port.

4. The microarray of claim 2, wherein the plurality of microchannels comprises a circumferential microchannel in fluid communication with a port such that aspiration of the microchannel can be performed via the port.

5. The microarray of claim 1, wherein the lattice of microchannels has a configuration selected from the group consisting of square and hexagonal.

6. The microarray of claim 1, wherein each of the microwell sets includes at least one microwell that is differentiated from other microwells in the microwell set.

7. The microarray of claim 6, wherein the at least one differentiated microwell is located at a predetermined location in each of the microwell sets such that an orientation of the microwell sets can be determined by visual inspection.

8. The microarray of claim 6, wherein the at least one differentiated microwell indicates information that can be used to determine a location of each of the microwell sets in the microarray.

9. The microarray of claim 8, wherein the information is a column and row number.

10. The microarray of claim 6, wherein the at least one differentiated microwell is a different shape from the other microwells in the microwell set.

11. The microarray of claim 1, wherein each of the microwells is substantially 50 μ

m by 50 μ

m by 50 μ

m.

12. The microarray of claim 1, dimensioned and configured to be compatible with 25 mm×

60 mm coverslips and standard glass slides used for microscopy.

13. The microarray of claim 1, wherein each of the microwell sets is sized to match the field of view available to a CCD used in a camera.

14. The microarray of claim 13, wherein the CCD is 1360×

1024 pixels.

15. The microarray of claim 1, wherein each microwell is sized to minimize the number of cells per microwell.

16. The microarray of claim 1, wherein the microchannels and microwells are arranged and constructed such that, when a cell-containing suspension is loaded onto the microarray, cells from the suspension settle into microwells in the microarray, and, when the planar surface of the moldable slab is placed in contact with a planar surface of a substrate such that a seal is formed between the moldable slab and the substrate, liquid on the planar surface of the moldable slab is excluded from an interfacial region, via the microchannels, and sufficient liquid is retained in each of the microwells after the seal has been achieved that cells contained within the microwells are not damaged or killed.