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Stabilized fibronectin domain compositions, methods and uses

  • US 8,569,227 B2
  • Filed: 04/29/2011
  • Issued: 10/29/2013
  • Est. Priority Date: 04/30/2010
  • Status: Active Grant
First Claim
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1. A method of constructing a library of a scaffold-based protein derived from a stability enhanced consensus sequence of a fibronectin type III domain incorporating randomized codons in order to produce polypeptide variants, comprising the steps of:

  • providing a polynucleotide sequence encoding a polypeptide comprising a stability enhanced consensus sequence of a fibronectin type III domain selected from the group consisting of SEQ ID NOs;

    142-151;

    introducing randomizing codons into the polynucleotide sequence at selected positions; and

    propagating copies of the polynucleotide sequence to form a library of polynucleotides encoding variant scaffold proteins.

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