Method for imaging and differential analysis of cells
First Claim
Patent Images
1. A method for distinguishing late apoptotic cells from necrotic cells, comprising:
- based on images of a population of cells, identifying a group of cells that are either of a necrotic type or a late apoptotic type; and
grouping cells in the population into a group of late apoptotic cells and a group of necrotic cells based on a brightfield image area of the cells and a darkfield peak intensity area of the cells, as determined from the images of the cells, where cells are identified as being in the group of late apoptotic cells if the cells have both a low brightfield area and a high darkfield peak intensity, and as being in the group of necrotic cells if the cells have both a high brightfield area and a low darkfield peak intensity.
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Abstract
Provided are methods for determining and analyzing photometric and morphometric features of small objects, such as cells to, for example, identify different cell states. In particularly, methods are provided for identifying apoptotic cells, and for distinguishing between cells undergoing apoptosis versus necrosis.
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6 Claims
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1. A method for distinguishing late apoptotic cells from necrotic cells, comprising:
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based on images of a population of cells, identifying a group of cells that are either of a necrotic type or a late apoptotic type; and grouping cells in the population into a group of late apoptotic cells and a group of necrotic cells based on a brightfield image area of the cells and a darkfield peak intensity area of the cells, as determined from the images of the cells, where cells are identified as being in the group of late apoptotic cells if the cells have both a low brightfield area and a high darkfield peak intensity, and as being in the group of necrotic cells if the cells have both a high brightfield area and a low darkfield peak intensity. - View Dependent Claims (2, 3, 4, 5, 6)
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Specification