Selective heated extraction of albumin proteins from intact freshwater algal cells
First Claim
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1. A method of selectively removing albumin proteins from a wet freshwater algal biomass comprised of intact algal cells or wet freshwater algal culture comprised of intact algal cells, the method comprising:
- a. providing a wet freshwater algal biomass comprised of intact algal cells or wet freshwater algal culture comprised of intact algal cells;
b. heating and mixing the wet freshwater algal biomass comprised of intact algal cells or wet freshwater algal culture under a pressure greater than or equal to atmospheric pressure to generate an extraction mixture comprising a heated liquid fraction and a heated biomass fraction comprised of intact algal cells; and
c. separating the heated liquid fraction containing albumin proteins from the heated biomass fraction comprised of intact algal cells, wherein the heated algal biomass fraction comprises lipids.
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Abstract
Methods for selective extraction and fractionation of algal proteins from an algal biomass or algal culture are disclosed. A method of selective removal of products from an algal biomass provides for single and multistep extraction processes which allow for efficient separation of algal proteins. These proteins can be used as renewable sources of proteins for animal feedstocks and human food. Further, lipids remaining in the algal biomass after extraction of proteins can be used to generate renewable fuels.
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11 Claims
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1. A method of selectively removing albumin proteins from a wet freshwater algal biomass comprised of intact algal cells or wet freshwater algal culture comprised of intact algal cells, the method comprising:
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a. providing a wet freshwater algal biomass comprised of intact algal cells or wet freshwater algal culture comprised of intact algal cells; b. heating and mixing the wet freshwater algal biomass comprised of intact algal cells or wet freshwater algal culture under a pressure greater than or equal to atmospheric pressure to generate an extraction mixture comprising a heated liquid fraction and a heated biomass fraction comprised of intact algal cells; and c. separating the heated liquid fraction containing albumin proteins from the heated biomass fraction comprised of intact algal cells, wherein the heated algal biomass fraction comprises lipids. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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