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Scaffolded nucleic acid polymer particles and methods of making and using

  • US 8,574,835 B2
  • Filed: 05/24/2010
  • Issued: 11/05/2013
  • Est. Priority Date: 05/29/2009
  • Status: Active Grant
First Claim
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1. A method of detecting a nucleotide incorporation, comprising:

  • (a) disposing a particle in a reaction chamber including, or capacitively coupled to, a field effect transistor, wherein the particle includes a hydrophillic non-nucleosidic polymer network and a plurality of nucleic acids attached to the non-nucleosidic polymer network, the particle having a coefficient of variance of volume of not greater than 15%, the non-nucleosidic polymer network includes a polyacrylamide gel having a total monomer percentage in a range of 3% to 20% and being permeable to proteins having a size in the range of 50 kilodaltons to 200 kilodaltons;

    (b) performing a nucleotide incorporation whereby a nucleotide is incorporated into one or more of the plurality of nucleic acids attached to the polymer network of the particle, and wherein the particle includes a sufficiently high concentration of attached nucleic acids to emit, upon such performing, a pH signal that can be detected by the field effect transistor; and

    (c) detecting the pH signal using the field effect transistor, thereby detecting the nucleotide incorporation.

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