×

Method for production of mesenchymal cell, method for production of tooth, and mesenchymal cell for formation of tooth

  • US 8,574,904 B2
  • Filed: 01/18/2008
  • Issued: 11/05/2013
  • Est. Priority Date: 01/22/2007
  • Status: Expired due to Fees
First Claim
Patent Images

1. A method for producing a tooth comprising:

  • culturing mouse or human pluripotent stem cells in the presence of a differentiation inducer to produce a cell population after differentiation induction treatment, said cell population containing CD44-positive and CD29-positive cells and/or CD44-positive and CD106-positive cells;

    selecting, from said cell population after the differentiation induction treatment, the CD44-positive and CD29-positive cells and/or the CD44-positive and CD106-positive cells as mesenchymal cells for the formation of the tooth, or obtaining the CD44-positive and CD29-positive cells and/or the CD44-positive and CD106-positive cells cultured from mouse or human pluripotent stem cells in the presence of a differentiation inducer;

    positioning, in a support carrier, a first cell mass consisting essentially of only either one of the mesenchymal cells or epithelial cells derived from a tooth germ and a second cell mass consisting essentially of only the other one of the mesenchymal cells or the epithelial cells derived from a tooth germ, said first and second cell masses being not mixed with each other but made to closely contact with each other; and

    culturing said first and second cell masses in said support carrier.

View all claims
  • 1 Assignment
Timeline View
Assignment View
    ×
    ×