Compositions and methods for separating, characterizing and administering soluble selenoglycoproteins

• US 8,575,320 B2
• Filed: 09/16/2011
• Issued: 11/05/2013
• Est. Priority Date: 03/18/2010
• Status: Active Grant

• Current Assignee: Alltech Incorporated (WR Grace & Company)
• Original Assignee: Alltech Incorporated (WR Grace & Company)
• Inventors: Kwiatkowski, Stefan, Power, Ronan, Matney, Clayton, Ghoroghchian, Paiman Peter, Ostertag, Eric Michael
• Primary Examiner(s): NOAKES, SUZANNE MARIE
• Application Number:
  US13/235,191
• Publication Number:
  US 20120164234A1
• Time in Patent Office: 781 Days
• Field of Search: None
• US Class Current: 530/395
• CPC Class Codes: A61K 36/064 Saccharomycetales, e.g. baker's yeast
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61K 9/1273 Polymersomes; Liposomes with polymerisable or polymerised bilayer-forming substances polymers grafted or coated on phosphatidyl liposomes A61K9/1271, on non-phosphatidyl liposomes A61K9/1272
• CPC Class Codes: C07K 14/39 from yeasts

Abstract

The invention relates to soluble selenium compositions and methods of production, separation and purification thereof. In particular the present invention provides methods of preparing water soluble selenoglycoproteins (e.g., via extracting selenoglycoproteins from selenium enriched yeast), methods of supplementing a selenium deficient composition via admixing water soluble selenoglycoproteins with the selenium deficient composition, compositions comprising the water soluble selenoglycoproteins and methods of administering the same.

Claims

1. A composition comprising selenoglycoproteins, wherein the selenoglycoproteins contain one or more isolated fractions of acid-soluble selenoglycoproteins present in a liquid phase having a pH between 1.0 and 6.5 comprising the extract of selenium enriched yeast obtained from acidic hydrolysis of the selenium enriched yeast.

2. The composition of claim 1, wherein the one or more isolated fractions of acid-soluble selenoglycoproteins are obtained, subsequent to acidic hydrolysis of the selenium enriched yeast, via pH dependent precipitation of the selenoglycoproteins.

3. The composition of claim 2, wherein pH dependent precipitation comprises raising the pH to a level at which a portion of the selenoglycoproteins precipitate out of the liquid phase having a pH between 1.0 and 6.5 comprising the extract of selenium enriched yeast soluble under acidic conditions and collecting the precipitated selenoglycoproteins.

4. The composition of claim 3, wherein the pH level is raised to 4.0.

5. The composition of claim 3, wherein the pH level is raised to 6.0.

6. The composition of claim 1, wherein the selenoglycoproteins contain a single fraction of selenoglycoproteins recovered from the liquid phase having a pH between 1.0 and 6.5 comprising the extract of acid hydrolyzed selenium enriched yeast.

7. The composition of claim 6, wherein the single fraction of selenoglycoproteins precipitates at pH 3.0 from the liquid phase having a pH between 1.0 and 6.5 comprising the extract of acid hydrolyzed selenium enriched yeast.

8. The composition of claim 6, wherein the single fraction of selenoglycoproteins precipitates at pH 4.0 from the liquid phase having a pH between 1.0 and 6.5 comprising the extract of acid hydrolyzed selenium enriched yeast.

9. The composition of claim 6, wherein the single fraction of selenoglycoproteins precipitates at pH 6.0 from the liquid phase having a pH between 1.0 and 6.5 comprising the extract of acid hydrolyzed selenium enriched yeast.

10. The composition of claim 1, wherein the selenoglycoproteins contain two separate fractions of selenoglycoproteins recovered from the liquid phase having a pH between 1.0 and 6.5 comprising the extract of acid hydrolyzed selenium enriched yeast.

11. A composition comprising selenoglycoproteins and a carrier, wherein the selenoglycoproteins contain one or more isolated fractions of acid-soluble selenoglycoproteins present in a liquid phase having a pH between 1.0 and 6.5 comprising the extract of selenium enriched yeast obtained from acidic hydrolysis of the selenium enriched yeast.

12. The composition of claim 11, wherein the carrier is selected from the group consisting of a polymersome, a slow-release polymer, a nanocapsule, and a molecularly imprinted polymer.

13. The composition of claim 11, wherein the carrier is a slow-release polymer.

14. The composition of claim 11, wherein the carrier is a polymersome used to encapsulate selenoglycoprotein.

15. The composition of claim 14, wherein the polymersome comprises poly(ethylene oxide) (PEO) block copolymer.

16. The composition of claim 14, wherein the polymersome comprises poly(ε

-caprolactone) (PCL) diblock copolymer.

17. The composition of claim 14, wherein the polymersome comprises poly(ethylene oxide)-block-poly(ε

-caprolactone) (PEO-b-PCL) based diblock copolymers.

18. The composition of claim 14, wherein the polymersome is derived from the coupling of poly(lactic acid), poly(glycolide), poly(lactic-coglycolic acid) or poly(3-hydroxybutyrate) with PEO.

19. The composition of claim 14, wherein the average diameter of a polymersome encapsulating selenoglycoprotein is 50-300 nm.