Synthesis of long-chain polyunsaturated fatty acids by recombinant cell
DC CAFCFirst Claim
1. A process for producing oil containing eicosapentaenoic acid and docosapentaenoic acid, comprising the steps of obtaining a transgenic seed of an oilseed plant which comprises eicosapentaenoic acid and docosapentaenoic acid in an esterified form as part of triglycerides, and a microalgal fatty acid desaturase, wherein the total fatty acid of the transgenic seed comprises at least 2.5% ω
- 3 C20 fatty acids (w/w) and wherein the docosapentaenoic acid is present at a level based on a conversion ratio of eicosapentaenoic acid to docosapentaenoic acid of at least 5% (w/w), and extracting oil from the transgenic seed so as to thereby produce the oil.
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Abstract
The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desaturase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids.
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20 Claims
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1. A process for producing oil containing eicosapentaenoic acid and docosapentaenoic acid, comprising the steps of obtaining a transgenic seed of an oilseed plant which comprises eicosapentaenoic acid and docosapentaenoic acid in an esterified form as part of triglycerides, and a microalgal fatty acid desaturase, wherein the total fatty acid of the transgenic seed comprises at least 2.5% ω
- 3 C20 fatty acids (w/w) and wherein the docosapentaenoic acid is present at a level based on a conversion ratio of eicosapentaenoic acid to docosapentaenoic acid of at least 5% (w/w), and extracting oil from the transgenic seed so as to thereby produce the oil.
- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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