Methods, devices, kits and compositions for detecting roundworm

• US 8,580,518 B2
• Filed: 05/18/2009
• Issued: 11/12/2013
• Est. Priority Date: 05/19/2008
• Status: Active Grant

• Current Assignee: Monsanto Company (Bayer AG), IDEXX Laboratories Incorporated
• Original Assignee: Divergence, Inc. (Bayer AG), IDEXX Laboratories Incorporated
• Inventors: Elsemore, David Allen, Flynn, Laurie A., Geng, Jinming, Crawford, Michael
• Primary Examiner(s): GRASER, JENNIFER E
• Application Number:
  US12/467,808
• Publication Number:
  US 20090286229A1
• Time in Patent Office: 1,639 Days
• Field of Search: None
• US Class Current: 435/7.1
• CPC Class Codes: C07K 14/4354 from nematodes
• CPC Class Codes: C07K 16/18 against material from animals or humans
• CPC Class Codes: C12Q 1/6888 for detection or identification of organisms
• CPC Class Codes: G01N 33/5308 for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
• CPC Class Codes: G01N 33/543 with an insoluble carrier for immobilising immunochemicals

Abstract

Methods, devices, kits and compositions for detecting the presence or absence of roundworm in a fecal sample are disclosed herein. The methods, devices, kits and compositions of the present invention may be used to confirm the presence or absence of roundworm in a fecal sample from a mammal that may also be infected with one or more of hookworm, whipworm, and heartworm. Confirmation of the presence or absence of roundworm in the mammal may be made, for example, for the purpose of selecting an optimal course of treating the mammal and/or for the purpose of determining whether the mammal has been rid of the infection after treatment has been initiated.

Claims

1. A method of detecting the presence or absence of one or more roundworm antigens in a sample, the method comprising the steps of:

(a) contacting a sample from a mammal with one or more antibodies that specifically bind to a polypeptide consisting of the amino acid sequence SEQ ID NO;

3, SEQ ID NO;

4, SEQ ID NO;

5, SEQ ID NO;

6, SEQ ID NO;

7, SEQ ID NO;

11, or Copro6728;

(b) forming antibody-polypeptide complexes in the presence of the polypeptides if any, in the sample; and

(c) detecting the presence or absence of the antibody-polypeptide complexes, if any.

3. The method of any of claim 1 or 2, further comprising the step of contacting the sample with one or more reagents to detect one or more of the group consisting of:

one or more non-roundworm worm parasites, one or more non-worm parasites, one or more viruses, one or more fungi, and one or more bacteria.

4. The method of claim 3, wherein the reagents for the detection of any one or all of the one or more non-roundworm worm parasites, one or more non-worm parasites, one or more viruses, one or more fungi and the one or more bacteria are one or more antibodies or one or more antigens recognized by antibodies specific for one or more non-roundworm worm parasites, one or more non-worm parasites, one or more viruses, one or more fungi or one or more bacteria.

5. The method of any of claim 1 or 2, wherein the one or more roundworm antigens are the polypeptide having amino acid sequence that corresponds to SEQ ID NO:

3, SEQ ID NO;

4, SEQ ID NO;

5, SEQ ID NO;

6, SEQ ID NO;

7, SEQ ID NO;

11, or Copro6728.

6. The method of any of claim 1 or 2, wherein the sample is obtained from a mammal that is a canine or a feline.

7. The method of claim 6, wherein the roundworm is Toxocara canis or Toxocara cati.

8. The method of any of claim 1 or 2, wherein the sample is a fecal sample.

9. The method of claim 8, wherein the one or more antibodies do not specifically bind any coproantigen derived from the group consisting of hookworm, whipworm, and heartworm.

10. The method of any of claim 1 or 2, wherein the step of detecting the presence or absence of the complexes further includes the step of providing at least one secondary antibody that binds to at least one of the complexes.

11. The method of claim 10, wherein the at least one secondary antibody is labeled.

12. The method of any of claim 1 or 2, wherein one or more of the one or more antibodies are labeled.

13. The method of any of claim 1 or 2, wherein the one or more antibodies are immobilized on a solid support.

14. The method of claim 13, wherein the solid support forms part of an enzyme-linked immunosorbent assay device.

15. The method of claim 14, wherein the enzyme-linked immunosorbent assay device is a lateral flow immunoassay device.

2. A method of diagnosing whether a mammal is infected with an intestinal roundworm infection, the method comprising the steps of:

(a) contacting a sample from a mammal with one or more antibodies that specifically bind to a polypeptide consisting of the amino acid sequence SEQ ID NO;

3, SEQ ID NO;

4, SEQ ID NO;

5, SEQ ID NO;

6, SEQ ID NO;

7, SEQ ID NO;

11, or Copro6728;

(b) forming antibody-polypeptide complexes in the presence of the polypeptides, if any, in the sample;

(c) detecting the presence or absence of the antibody-polypeptide complexes, if any; and

(d) diagnosing the mammal as having the roundworm infection if a roundworm antibody-polypeptide complex is present.