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Phospholink nucleotides for sequencing applications

  • US 8,580,539 B2
  • Filed: 12/31/2012
  • Issued: 11/12/2013
  • Est. Priority Date: 11/17/2008
  • Status: Active Grant
First Claim
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1. A method of determining an identity of a nucleotide in a template nucleic acid sequence, said method comprising:

  • a. providing said template nucleic acid sequence complexed with a polymerase enzyme capable of template dependent synthesis of a complementary nascent sequence as a first complex;

    b. contacting said first complex with a labeled phospholink nucleotide, wherein said labeled phospholink nucleotide comprises a bead comprising at least one fluorophore, wherein said bead is encapsulated in an organic, inorganic, or polymeric material, and wherein said labeled phospholink nucleotide is complementary to a known nucleotide; and

    c. detecting whether said labeled phospholink nucleotide is incorporated into said nascent sequence, wherein incorporation of said labeled phospholink nucleotide is indicative that said complementary nucleotide is in a position in the template nucleic acid that is being processed by the polymerase enzyme.

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