Device with diaphragm valve

• US 8,584,703 B2
• Filed: 11/18/2010
• Issued: 11/19/2013
• Est. Priority Date: 12/01/2009
• Status: Active Grant

• Current Assignee: IntegenX Inc. (Thermo Fisher Scientific Incorporated)
• Original Assignee: IntegenX Inc. (Thermo Fisher Scientific Incorporated)
• Inventors: Kobrin, Boris, Blaga, Iuliu I., Nielsen, William, Qi, Shize D., Van Gelder, Ezra
• Primary Examiner(s): Fristoe, Jr., John K
• Assistant Examiner(s): Sanchez-Medina, Reinaldo
• Application Number:
  US12/949,623
• Publication Number:
  US 20110126911A1
• Time in Patent Office: 1,097 Days
• Field of Search: 137/597, 251/61
• US Class Current: 137/597
• CPC Class Codes: B01F 31/65 the materials to be mixed being directly submitted to a pulsating movement, e.g. by means of an oscillating piston or air column
• CPC Class Codes: B01F 33/30 Micromixers
• CPC Class Codes: B32B 7/022 Mechanical properties
• CPC Class Codes: B32B 9/04 comprising such particular substance as the main or only constituent of a layer, which is next to another layer of the same or of a different material next to a glass layer B32B17/06; layered products with at least two ceramic layers composed mainly of ceramic B32B18/00
• CPC Class Codes: B33Y 80/00 Products made by additive manufacturing
• CPC Class Codes: F16K 2099/008 Multi-layer fabrications
• CPC Class Codes: F16K 2099/0084 Chemistry or biology, e.g. "lab-on-a-chip" technology
• CPC Class Codes: F16K 2099/0094 Micropumps
• CPC Class Codes: F16K 99/0001 Microvalves microdevices B81B1/00; manufacture or treatment of devices or systems in or on a substrate B81C1/00; microfluidic structures B01L3/5027; micropumps F04B19/006
• CPC Class Codes: F16K 99/0015 Diaphragm or membrane valves
• CPC Class Codes: F16K 99/0059 actuated by a pilot fluid
• CPC Class Codes: Y10T 137/0318 Processes
• CPC Class Codes: Y10T 137/2213 Electrically-actuated element [e.g., electro-mechanical transducer]
• CPC Class Codes: Y10T 137/86453 Plural trips or trip actuations
• CPC Class Codes: Y10T 137/87249 Multiple inlet with multiple outlet
• CPC Class Codes: Y10T 156/1039 Surface deformation only of sandwich or lamina [e.g., embossed panels]
• CPC Class Codes: Y10T 428/31504 Composite [nonstructural laminate]

Abstract

This invention provides composite plastic articles and methods of making them. The articles can be fluidic or microfluidic devices having fluidic conduits and, optionally, pneumatic conduits that regulate flow in the fluidic conduits. The articles comprise a first substrate coated with a layer of a material that comprises, or onto which have been introduced, reactive groups. For example, the substrate can be a plastic coated with an oxide or a siloxane onto which hydroxyl groups have been introduced. These articles are covalently bonded with other articles comprising reactive groups on their surfaces, for example, polysiloxanes treated to have silanol groups. Certain articles have specified locations on their surfaces that are not bonded to the other piece. For example, the coating can be removed from these locations before bonding. Such locations can be useful as functional elements of various devices, such as valve seats in valves of microfluidic devices.

Claims

1. A microfluidic device comprising a plurality of diaphragm valves configured to regulate fluid flow along each of a plurality of microfluidic channels and comprised in a combination that comprises a fluidics layer, an actuation layer and an elastic layer sandwiched between the fluidics layer and the actuation layer,

wherein each diaphragm valve comprises:

a) a diaphragm comprised in the elastic layer;

b) a valve seat comprised in the fluidics layer and recessed from a surface of the fluidics layer that contacts the elastic layer so that the diaphragm does not close the diaphragm valve unless positive pressure is exerted on the diaphragm; and

c) a valve inlet and a valve outlet comprised in the fluidics layer and in fluid communication with the microfluidic channel;

wherein both of the fluidics layer and the actuation layer comprise a plastic substrate coated with a material covalently bonded to the elastic layer and wherein the fluidics layer and the actuation layer are covalently bonded to the elastic layer through siloxy bonds and wherein the elastic layer is not bonded to all or part of the surface of the valve seat; and

wherein the diaphragm is configured to be actuated by positive or negative pressure transmitted to the diaphragm through an actuation conduit in the actuation layer and wherein one actuation conduit is configured to actuate a plurality of diaphragm valves in parallel.

2. The device of claim 1, wherein the valve seat has a concave surface with respect to the surface of the fluidics layer.

3. The device of claim 2, wherein the valve seat has a shape of a dome, a section of a sphere, or an inverted dimple.

4. The device of claim 3, wherein the elastic layer comprises a polysiloxane.

5. The device of claim 2, wherein the concave surface of the valve seat comprises at least one area which has a convex surface and meets the elastic layer when positive pressure is exerted on the diaphragm.

6. The device of claim 5, wherein the concave surface of the valve seat comprising at least one convex area has a shape of a saddle or an inverted dimple comprising an extraverted dimple.

7. The device of claim 1, wherein the actuation layer comprises a chamber which is disposed opposite the valve seat.

8. The device of claim 1, wherein the elastic layer comprises a polysiloxane.

9. The device of claim 8, wherein the polysiloxane is polydimethylsiloxane (PDMS).

10. The device of claim 1, wherein the fluidics layer or the actuation layer, or both, comprise a polymer selected from the group consisting of a carbon-based polymer, a cycloolefin polymer, a cycloolefin copolymer, an olefin copolymer, an acrylic, polymethylmethoxyacrylate (PMMA), a polypropylene, a polystyrene, a polycarbonate, a polythiol, and a liquid crystal polymer.

11. The device of claim 1, wherein the material coating the fluidics layer or the actuation layer comprises an oxide.

12. The device of claim 11, wherein the oxide is silicon oxide.

13. The device of claim 1, wherein the fluidics layer comprises a plurality of sublayers.

14. An instrument comprising the device of claim 1.

15. The instrument of claim 14 wherein the valve seat has a concave surface with respect to the surface of the fluidics layer, wherein the valve seat has a shape of a dome, a section of a sphere, or an inverted dimple.

16. A microfluidic device comprising a pump configured to regulate fluid flow along a microfluidic channel and comprised in a combination that comprises a fluidics layer, an actuation layer and an elastic layer sandwiched between the fluidics layer and the actuation layer, wherein the pump comprises, in series, a first diaphragm valve, a pumping valve, and a second diaphragm valve;

(I) wherein each diaphragm valve comprises;

a) a diaphragm comprised in the elastic layer;

b) a valve seat comprised in the fluidics layer and recessed from a surface of the fluidics layer that contacts the elastic layer so that the diaphragm does not close the diaphragm valve unless positive pressure is exerted on the diaphragm; and

c) a valve inlet and a valve outlet comprised in the fluidics layer and in fluid communication with the microfluidic channel; and

(II) wherein the pumping valve comprises;

a) a diaphragm comprised in the elastic layer;

b) a chamber comprised in the actuation layer and in the fluidics layer and recessed from a surface of the fluidics layer that contacts the elastic layer; and

c) a pumping valve inlet and a pumping valve outlet comprised in the fluidics layer and in fluid communication with the microfluidic channel;

wherein deforming the diaphragm of the pumping valve into the actuation chamber creates a volume to accept fluid, and deforming the diaphragm into the fluidics chamber pumps liquid out of the chamber and wherein the pumping valve does not completely block fluid flow in the microfluidic channel; and

wherein the diaphragms in the diaphragm valves and the pumping valve are configured to be actuated by positive or negative pressure transmitted to the diaphragm through an actuation conduit in the actuation layer, and wherein both of the fluidics layer and the actuation layer comprises a plastic substrate coated with a material covalently bonded to the elastic layer and wherein the fluidics layer and the actuation layer are covalently bonded to the elastic layer through siloxy bonds and wherein the elastic layer is not bonded to all or part of the surface of the valve seat.

17. The device of claim 16 comprising a plurality of said pumps, wherein a first actuation channel is configured to actuate the first diaphragm valve in each of the pumps in parallel, a second actuation channel is configured to actuate the second diaphragm valve in each of the pumps in parallel, and a third actuation channel is configured to actuate the pumping valve in each of the pumps in parallel.

18. The device of claim 17, wherein both of the fluidics layer and the actuation layer comprises a plastic substrate coated with a material covalently bonded to the elastic layer and wherein the fluidics layer and the actuation layer are covalently bonded to the elastic layer through siloxy bonds and wherein the elastic layer is not bonded to all or part of the surface of the valve seat.