Markers of definitive endoderm

• US 8,586,357 B2
• Filed: 11/13/2006
• Issued: 11/19/2013
• Est. Priority Date: 12/23/2003
• Status: Active Grant

• Current Assignee: Cynthera, Inc., Viacyte Incorporated (Vertex Pharmaceuticals Incorporated)
• Original Assignee: Viacyte Incorporated (Vertex Pharmaceuticals Incorporated)
• Inventors: D'Amour, Kevin Allen, Agulnick, Alan D, Baetge, Emmanuel Edward
• Primary Examiner(s): Singh, Anoop
• Assistant Examiner(s): Montanari, David A
• Application Number:
  US12/093,590
• Publication Number:
  US 20090220959A1
• Time in Patent Office: 2,563 Days
• Field of Search: 435/325, 435/366
• US Class Current: 435/366
• CPC Class Codes: A61K 35/38 Stomach; Intestine; Goblet cells; Oral mucosa; Saliva
• CPC Class Codes: A61K 35/407 Liver; Hepatocytes
• CPC Class Codes: C12N 2501/16 Activin; Inhibin; Mullerian inhibiting substance
• CPC Class Codes: C12N 2501/415 Wnt; Frizzeled
• CPC Class Codes: C12N 5/0603 Embryonic cells production of embryos, nuclear transfer A01K67/027; Embryoid bodies
• CPC Class Codes: C12N 5/0617 Thyroid and parathyroid glands
• CPC Class Codes: C12N 5/0672 Stem cells; Progenitor cells; Precursor cells; Oval cells
• CPC Class Codes: C12N 5/068 Stem cells; Progenitors

Abstract

Disclosed herein are reagent-cell complexes comprising one or more definitive endoderm cells. Also described herein are compositions for detecting definitive endoderm. Method of enriching, isolating and/or purifying definitive endoderm cells are also disclosed.

Claims

1. An isolated human cell population wherein at least 20% of cells in said cell population form an ex vivo reagent-cell complex comprising:

a human definitive endoderm cell expressing a marker selected from the group consisting of CALCR, CMKOR1, CXCR4, GPR37, NTN4, RTN4RL1, SLC5A9, SLC40A1, and TRPA1, said definitive endoderm cell being a multipotent cell that can differentiate into cells of the gut tube or organs derived therefrom; and

a reagent bound to said marker.

2. The reagent cell complex of claim 1, wherein said marker comprises a polypeptide selected from the group consisting of CXCR4, GPR37, NTN4, RTN4RL1, SLC40A1, SLC5A9 and TRPA1.

3. The human cell population of claim 1, wherein at least 40% of said human cells form said ex vivo reagent-cell complex.

4. The human cell population of claim 1, wherein at least 60% of said human cells form said ex vivo reagent-cell complex.

5. The human cell population of claim 1, wherein at least 80% of said human cells form said ex vivo reagent-cell complex.

6. An isolated human cell population wherein at least 20% of cells in said cell population form an ex vivo reagent-cell complex comprising:

a human definitive endoderm cell expressing CXCR4, said definitive endoderm cell being a multipotent cell that can differentiate into cells of the gut tube or organs derived therefrom and a reagent bound to CXCR4.

7. The human cell population of claim 6, wherein at least 40% of said human cells form said ex vivo reagent-cell complex complexes.

8. The human cell population of claim 6, wherein at least 60% of said human cells form said ex vivo reagent-cell complex.

9. The human cell population of claim 6, wherein at least 80% of said human cells form said ex vivo reagent-cell complex.