Method and apparatus for nanoparticle electrogenerated chemiluminescence amplification
DC CAFCFirst Claim
Patent Images
1. A method of analyzing a sample comprising:
- contacting a liquid sample with a working electrode;
wherein the liquid sample comprises a plurality or electrogenerated chemiluminescent (ECL) moieties and a colloidal suspension of one or more conductive or redox active nanoparticles; and
detecting one or more transient optical properties resulting from interaction of the one or more nanoparticles and the sample at the working electrode;
wherein the one or more transient optical properties comprise an ECL intensity vs. time transient resulting from redox reactions of the ECL moieties, with or without coreactants, catalyzed by an individual nanoparticle.
1 Assignment
Litigations
0 Petitions
Accused Products
Abstract
Methods, compositions and kits for analyzing a chemical analyte using an electrochemical cell connected to a measuring apparatus are provided. The electrochemical cell contains a solution having one or more conductive or redox active NPs (nanoparticles), one or more chemical analytes, and an indicator. In addition, the electrochemical cell contains one or more electrodes in communication with the solution. One or more catalytic ECL properties are generated by the interaction of the one or more conductive or redox active NPs and the liquid sample and measured at the one or more electrodes or with an optical detection system.
95 Citations
22 Claims
-
1. A method of analyzing a sample comprising:
-
contacting a liquid sample with a working electrode;
wherein the liquid sample comprises a plurality or electrogenerated chemiluminescent (ECL) moieties and a colloidal suspension of one or more conductive or redox active nanoparticles; anddetecting one or more transient optical properties resulting from interaction of the one or more nanoparticles and the sample at the working electrode; wherein the one or more transient optical properties comprise an ECL intensity vs. time transient resulting from redox reactions of the ECL moieties, with or without coreactants, catalyzed by an individual nanoparticle. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
-
-
19. A method of analyzing a sample comprising:
-
contacting a liquid sample with a working electrode, wherein the liquid sample comprises a plurality of electrogenerated chemiluminescent (ECL) moieties and one or more conductive or redox active nanoparticles; and detecting one or more optical properties resulting from interaction of the one or more nanoparticles and the sample at the working electrode; wherein the one or more optical properties result from redox reactions of the ECL moieties, with or without coreactants, catalyzed by the one or more nanoparticles; the nanoparticles are platinum nanoparticles having a largest dimension of no more than about 50 nm; the ECL moieties each comprise an electrochemiluminescent polydendate metal complex which includes a ruthenium ion; and the liquid sample further comprises an ECL coreactant which includes tripropyl amine or triethyl amine.
-
-
20. A method of analyzing a sample comprising:
-
contacting a liquid sample with a working electrode, wherein the liquid sample comprises a plurality of electrogenerated chemiluminescent (ECL) moieties and one or more conductive or redox active nanoparticles; and detecting one or more optical properties resulting from interaction of the one or more nanoparticles and the sample at the working electrode; wherein the one or more optical properties resulting from interaction of the one or more moieties, with or without coreactants, catalyzed by the one or more nanoparticles; and
detecting one or more optical properties comprises measuring ECL intensity vs. time transients resulting from redox reactions of the ECL moieties catalyzed by individual nanoparticles;the liquid sample further comprises an ECL coreactant; the working electrode surface is at a potential which does not produce electrogenerated chemiluminescent in the absence of the one or more nanoparticles; the one or more conductive or redox active nanoparticles comprise platinum nanoparticles or palladium nanoparticles; and the ECL moieties comprise an electrochemiluminescent polydendate metal complex which includes heteroaromatic polydentate ligands and ruthenium. - View Dependent Claims (21)
-
-
22. A method of analyzing a sample comprising:
-
contacting a liquid sample with a working electrode;
wherein the liquid sample comprises a plurality of electrogenerated chemiluminescent (ECL) moieties and one or more conductive or redox active nanoparticles; anddetecting one or more transient optical properties resulting from interaction of the one or more nanoparticles and the sample at the working electrode; wherein the one or more transient optical properties comprise transient optical properties resulting from redox reactions of the ECL moieties, with or without coreactants, catalyzed by the one or more nanoparticles; the one or more conductive or redox active nanoparticles comprise platinum nanoparticles or palladium nanoparticles; the ECL moieties comprise an electrochemiluminescent polydendate metal complex which includes heteroaromatic polydentate ligands and ruthenium; and the working electrode surface is at a potential which does not produce electrogenerated chemiluminescent in the absence of the one or more nanoparticles.
-
Specification