Western blot by incorporating an affinity purification zone
First Claim
1. A method of detecting a component of interest in a mixture of components, the method comprising:
- (i) flowing the mixture of components through a binding channel region, which binding channel region comprises a component-binding moiety, thereby binding at least a portion of the component of interest to the component-binding moiety;
(ii) flowing the mixture of components through a separation channel region, resulting in one or more separated components, wherein the separation channel region comprises a separation buffer, which buffer comprises a detergent;
(iii) mixing a first portion of diluent with the one or more separated components, thereby diluting the detergent;
(iv) detecting the one or more separated components;
(v) releasing the component of interest from the component-binding moiety, resulting in a released component of interest;
(vi) flowing the released component of interest through the separation channel region;
(vii) mixing a second portion of diluent with the released component of interest, thereby diluting the detergent; and
(viii) detecting the released component of interest.
2 Assignments
0 Petitions
Accused Products
Abstract
A mixture of components is flowed through a binding channel region comprising a component-binding moiety, thereby binding at least a portion of a component of interest. The mixture is then flowed through a separation channel region that includes a buffer comprising a detergent, resulting in separated components. Diluent is mixed with the separated components, diluting the detergent, and the separated components are detected. The component of interest is released from the component-binding moiety and flowed through the separation channel region. Diluent is mixed with the released component of interest, diluting the detergent, and the released component of interest is detected.
87 Citations
20 Claims
-
1. A method of detecting a component of interest in a mixture of components, the method comprising:
-
(i) flowing the mixture of components through a binding channel region, which binding channel region comprises a component-binding moiety, thereby binding at least a portion of the component of interest to the component-binding moiety; (ii) flowing the mixture of components through a separation channel region, resulting in one or more separated components, wherein the separation channel region comprises a separation buffer, which buffer comprises a detergent; (iii) mixing a first portion of diluent with the one or more separated components, thereby diluting the detergent; (iv) detecting the one or more separated components; (v) releasing the component of interest from the component-binding moiety, resulting in a released component of interest; (vi) flowing the released component of interest through the separation channel region; (vii) mixing a second portion of diluent with the released component of interest, thereby diluting the detergent; and (viii) detecting the released component of interest. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
-
Specification