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Western blot by incorporating an affinity purification zone

  • US 8,592,141 B2
  • Filed: 08/24/2011
  • Issued: 11/26/2013
  • Est. Priority Date: 06/05/2001
  • Status: Expired due to Term
First Claim
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1. A method of detecting a component of interest in a mixture of components, the method comprising:

  • (i) flowing the mixture of components through a binding channel region, which binding channel region comprises a component-binding moiety, thereby binding at least a portion of the component of interest to the component-binding moiety;

    (ii) flowing the mixture of components through a separation channel region, resulting in one or more separated components, wherein the separation channel region comprises a separation buffer, which buffer comprises a detergent;

    (iii) mixing a first portion of diluent with the one or more separated components, thereby diluting the detergent;

    (iv) detecting the one or more separated components;

    (v) releasing the component of interest from the component-binding moiety, resulting in a released component of interest;

    (vi) flowing the released component of interest through the separation channel region;

    (vii) mixing a second portion of diluent with the released component of interest, thereby diluting the detergent; and

    (viii) detecting the released component of interest.

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