Methods and apparatus for high speed operation of a chemically-sensitive sensor array
First Claim
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1. A method for operating a plurality of chemically-sensitive sensors, the method comprising:
- placing the plurality of sensors in a reference condition;
acquiring first reference data from the plurality of sensors at a first data rate that is equal to or lower than a reciprocal of a settling time of readout circuitry coupled to the sensors;
acquiring second reference data from the plurality of sensors at a second data rate that is faster than the first data rate;
determining a correction factor based on the first reference data and the second reference data;
exposing the plurality of sensors to an analyte;
acquiring sensor data from the plurality of sensors at the second data rate; and
applying the correction factor to the sensor data to produce data representative of a property of the analyte.
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Abstract
Methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.
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Citations
8 Claims
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1. A method for operating a plurality of chemically-sensitive sensors, the method comprising:
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placing the plurality of sensors in a reference condition; acquiring first reference data from the plurality of sensors at a first data rate that is equal to or lower than a reciprocal of a settling time of readout circuitry coupled to the sensors; acquiring second reference data from the plurality of sensors at a second data rate that is faster than the first data rate; determining a correction factor based on the first reference data and the second reference data; exposing the plurality of sensors to an analyte; acquiring sensor data from the plurality of sensors at the second data rate; and applying the correction factor to the sensor data to produce data representative of a property of the analyte. - View Dependent Claims (2, 3)
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4. A method for operating an array of sensors, each sensor in the array including a chemically-sensitive field effect transistor, the method comprising:
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placing sensors in the array in a reference condition; acquiring reference data from the array of sensors at a first data rate and at a second data rate faster than the first data rate; exposing the plurality of sensors to an analyte; acquiring sensor data from the array of sensors at a sensing rate faster than the first data rate; and applying a correction factor to the sensor data based on said acquired reference data to produce data representative of an analyte property. - View Dependent Claims (5, 6, 7, 8)
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Specification