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Microfabricated crossflow devices and methods

  • US 8,592,215 B2
  • Filed: 09/29/2011
  • Issued: 11/26/2013
  • Est. Priority Date: 09/15/2000
  • Status: Expired due to Term
First Claim
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1. A method for sorting particles in a microfluidic device, the method comprising:

  • (a) flowing a sample fluid containing particles through a first channel while flowing an extrusion fluid immiscible with the sample fluid through a second channel towards a junction of the two channels that is configured so that droplets of the sample fluid are introduced into the extrusion fluid;

    (b) flowing the droplets formed in step (a) through a detection region that is downstream from the junction;

    (c) interrogating particle(s) in each droplet while the droplet is flowing through the detection region; and

    (d) directing each droplet into a branched channel that has been selected from two or more branched channels depending on results of the interrogating in step (c).

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