Immunostimulatory oligonucleotides and use thereof in pharmaceuticals
First Claim
1. An artificial immunostimulatory oligonucleotide comprising:
- the nucleotide sequence represented by the formula;
5′
-(G)MPXCGYQ(G)N-3′
(SEQ ID NO.;
117) wherein,C is cytosine;
G is guanine;
X and Y are mutually independent and each represent an arbitrary sequence which has a length of 0 to 10 nucleotides and does not contain 4 or more consecutive guanine residues, and the entire length of X+Y is 6 to 20 nucleotides;
XCGY contains a palindrome sequence having a length of at least 8 nucleotides and XCGY has a length of 8 to 22 nucleotides;
P and Q are mutually independent and each represent one nucleotide other than guanine,M represents an integer of 6 to 10,N represents an integer of 0 to 3;
and the length of X and Y may be the same or different, andwherein the length of the artificial immunostimulatory oligonucleotide is 16 to 37 nucleotides in total,wherein the palindrome sequence contains CGATCG (SEQ ID NO;
59), ATCGAT (SEQ ID NO;
60), or GACGTC (SEQ ID NO;
61),but the artificial immunostimulatory nucleotide is not SEQ ID NO;
5 GGGGGGTGCCGATCGGCAGGG.
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Accused Products
Abstract
A novel immunostimulatory oligonucleotide by which an IFN-inducing activity is enhanced and an inflammatory cytokine-inducing activity is reduced, and a pharmaceutical containing the same, and an application thereof are provided. That is, the present invention provides the immunostimulatory oligonucleotide composed of a base sequence represented by a formula: 5′-(G)MPXCGYQ(G)N-3′ (SEQ ID NO: 118) (X and Y are mutually independent and represent an arbitrary sequence which has a length of 0 to 10 nucleotides and does not contain 4 or more consecutive G residues, and a length of X+Y is 6 to 20 nucleotides; XCGY contains a palindrome sequence having a length of at least 8 nucleotides and has a length of 8 to 22 nucleotides; P and Q are mutually independent and represent one nucleotide other than G; M represents an integer of 6 to 10 and N represents an integer of 0 to 3) wherein a full length thereof is 16 to 37 nucleotides (except for an oligonucleotide composed of a base sequence represented by SEQ ID NO:5), the pharmaceutical application thereof.
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Citations
16 Claims
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1. An artificial immunostimulatory oligonucleotide comprising:
-
the nucleotide sequence represented by the formula;
5′
-(G)MPXCGYQ(G)N-3′
(SEQ ID NO.;
117) wherein,C is cytosine; G is guanine; X and Y are mutually independent and each represent an arbitrary sequence which has a length of 0 to 10 nucleotides and does not contain 4 or more consecutive guanine residues, and the entire length of X+Y is 6 to 20 nucleotides; XCGY contains a palindrome sequence having a length of at least 8 nucleotides and XCGY has a length of 8 to 22 nucleotides; P and Q are mutually independent and each represent one nucleotide other than guanine, M represents an integer of 6 to 10, N represents an integer of 0 to 3; and the length of X and Y may be the same or different, and wherein the length of the artificial immunostimulatory oligonucleotide is 16 to 37 nucleotides in total, wherein the palindrome sequence contains CGATCG (SEQ ID NO;
59), ATCGAT (SEQ ID NO;
60), or GACGTC (SEQ ID NO;
61),but the artificial immunostimulatory nucleotide is not SEQ ID NO;
5 GGGGGGTGCCGATCGGCAGGG. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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Specification