Elite event EE-GM3 and methods and kits for identifying such event in biological samples

• US 8,592,650 B2
• Filed: 11/23/2010
• Issued: 11/26/2013
• Est. Priority Date: 11/23/2009
• Status: Active Grant

• Current Assignee: BASF Agricultural Solutions Seed US LLC (BASF SE), MS Technologies LLC
• Original Assignee: Bayer Cropscience NV (Bayer AG), MS Technologies LLC
• Inventors: Mason, Justin Thomas, Lettow, Leslie James, Eby, Mark Alan, Eby, William H., Verhaeghe, Steven, De Beuckeleer, Marc, Habex, Veerle, Ferrulo, Jean-Marc, Welz, Gnter
• Primary Examiner(s): Kruse, David H
• Assistant Examiner(s): KOVALENKO, MYKOLA V
• Application Number:
  US12/953,212
• Publication Number:
  US 20110162098A1
• Time in Patent Office: 1,099 Days
• Field of Search: 800/300
• US Class Current: 800/300
• CPC Class Codes: C12N 15/8274 for herbicide resistance
• CPC Class Codes: C12N 15/8275 Glyphosate
• CPC Class Codes: C12Q 1/6895 for plants, fungi or algae
• CPC Class Codes: C12Q 2600/13 Plant traits
• CPC Class Codes: C12Q 2600/156 Polymorphic or mutational markers

Abstract

The invention provides specific transgenic soybean plants, plant material and seeds, characterized in that these products harbor a specific herbicide tolerance transformation event at a specific location in the soybean genome (elite event EE-GM3). The invention also provides for methods of producing soybean plants and seeds having elite event EE-GM3.

Claims

1. A transgenic soybean plant, or cells, part, seed or progeny thereof, each comprising elite event EE-GM3 in its genome, reference seed comprising said event having been deposited at the NCIMB under deposit number NCIMB 41659.

2. The transgenic soybean plant, seed, cells, part or progeny of claim 1, the genomic DNA of which, when analyzed by PCR with two primers comprising the nucleotide sequence of SEQ ID No. 4 and SEQ ID No. 5 respectively, yields a DNA fragment of about 263 bp.

8. A method for producing a soybean plant or seed each comprising elite event EE-GM3 comprising crossing the plant according to claim 1 with another soybean plant, and planting the seed obtained from said cross.

9. Soybean genomic DNA comprising the elite event EE-GM3 of claim 1.

16. A method for producing a soybean plant or seed comprising elite event EE-GM3, said method comprising crossing the plant according to claim 2 with another soybean plant, and planting the seed comprising elite event EE-GM3 obtained from said cross.

21. A soybean product produced from the seed of claim 1, wherein said soybean product comprises elite event EE-GM3 and is soybean meal, ground seeds, soybean flour, or soybean flakes.

26. A method for producing a soybean plant tolerant to glyphosate and/or isoxaflutole herbicides, comprising introducing into the genome of said soybean plant elite event EE-GM3 of claim 1, and selecting a progeny plant tolerant to glyphosate and/or isoxaflutole, wherein said progeny plant comprises elite event EE-GM3.

27. The method of claim 26, wherein said introducing comprises crossing a first soybean plant lacking elite event EE-GM3 with a soybean plant comprising elite event EE-GM3, and selecting a progeny plant tolerant to glyphosate and/or isoxaflutole, wherein said progeny plant comprises elite event EE-GM3.

38. A method for controlling weeds in a field of the soybean plant of claim 1, comprising treating the field with an effective amount of an isoxaflutole-based herbicide, wherein said soybean plant is tolerant to said isoxaflutole-based herbicide.

39. A method for controlling weeds in a field of the soybean plants or seeds of claim 1, comprising treating a field with an HPPD inhibitor herbicide, before said soybean plants are planted or the seeds are sown, followed by planting or sowing of said soybeans in that same pre-treated field.

40. A method for controlling weeds in a field of the soybean seeds of claim 1, comprising treating a field in which said seeds were sown, with an HPPD inhibitor herbicide before the soybean plants emerge but after the seeds are sown.

41. A method for controlling weeds in a field of the soybean plants of claim 1, comprising treating said plants with an HPPD inhibitor herbicide over the top of the soybean plants, which application can be together with, followed by or preceded by a treatment with glyphosate as post-emergent herbicide over the top of the plants.

3. Seed comprising elite event EE-GM3 deposited at the NCIMB under deposit number NCIMB 41659 or progeny seed comprising elite event EE-GM3 obtainable from said seed.

4. A soybean plant, plant part, cell, or tissue, or seed each comprising elite event EE-GM3 obtainable from the seed of claim 3.

17. A method for producing a soybean plant or seed comprising elite event EE-GM3, said method comprising crossing the plant according to claim 4 with another soybean plant, and planting the seed comprising elite event EE-GM3 obtained from said cross.

22. A soybean product produced from the seed of claim 4, wherein said soybean product comprises elite event EE-GM3 and is soybean meal, ground seeds, soybean flour, or soybean flakes.

28. A method for producing a soybean plant tolerant to glyphosate and/or isoxaflutole herbicides, comprising crossing a first soybean plant lacking elite event EE-GM3 with the soybean plant comprising elite event EE-GM3 of claim 4, and selecting a progeny plant tolerant to glyphosate and/or isoxaflutole, wherein said progeny plant comprises elite event EE-GM3.

5. A soybean plant, or seed, cells or tissues thereof, each comprising elite event EE-GM3 in its genome, obtainable by propagation of and/or breeding with a soybean plant grown from the seed deposited at the NCIMB under deposit number NCIMB 41659.

18. A method for producing a soybean plant or seed comprising elite event EE-GM3, said method comprising crossing the plant according to claim 5 with another soybean plant, and planting the seed comprising elite event EE-GM3 obtained from said cross.

23. A soybean product produced from the seed of claim 5, wherein said soybean product comprises elite event EE-GM3 and is soybean meal, ground seeds, soybean flour, or soybean flakes.

29. A method for producing a soybean plant tolerant to glyphosate and/or isoxaflutole herbicides, comprising crossing a first soybean plant lacking elite event EE-GM3 with the soybean plant comprising elite event EE-GM3 of claim 5, and selecting a progeny plant tolerant to glyphosate and/or isoxaflutole, wherein said progeny plant comprises elite event EE-GM3.

6. A soybean seed comprising elite event EE-GM3, reference seed comprising said event having been deposited at the NCIMB under deposit number NCIMB 41659.

7. A transgenic soybean plant, cell, or tissue, each comprising elite event EE-GM3, obtainable from the seed of claim 6.

19. A method for producing a soybean plant or seed comprising elite event EE-GM3, said method comprising crossing the plant according to claim 7 with another soybean plant, and planting the seed comprising elite event EE-GM3 obtained from said cross.

10. A soybean plant, comprising in its genome elite event EE-GM3, wherein said event comprises in order the following nucleotide sequences:

a) the nucleotide sequence of SEQ ID No. 2 from nucleotide 1 to 1451;

b) the nucleotide sequence of the complement of the nucleotide sequence of SEQ ID 1 from nucleotide 6760 to nucleotide 6958;

c) the nucleotide sequence of SEQ ID No. 1 from nucleotide 6874 to nucleotide 7298;

d) the nucleotide sequence of SEQ ID No. 1 from nucleotide 7 to nucleotide 7291;

e) the nucleotide sequence of SEQ ID No. 1 from nucleotide 12 to nucleotide 7265;

f) the nucleotide sequence of SEQ ID No. 3 from nucleotide 217 to nucleotide 240; and

g) the nucleotide sequence of SEQ ID No. 3 from nucleotide 241 to nucleotide 1408.

30. A method for producing a soybean plant tolerant to glyphosate and/or isoxaflutole herbicides, comprising crossing a first soybean plant lacking elite event EE-GM3 with the soybean plant comprising elite event EE-GM3 of claim 10, and selecting a progeny plant tolerant to glyphosate and/or isoxaflutole, wherein said progeny plant comprises elite event EE-GM3.

11. A transgenic soybean plant, plant cell, tissue, or seed, comprising in its genome elite event EE-GM3, wherein said event comprises a nucleic acid sequence of SEQ ID No. 2 from nucleotide 1441 to nucleotide 1462 and a nucleic acid sequence of SEQ ID No. 3 from nucleotide 230 to 251, or the complement of said sequences.

20. A method for producing a soybean plant or seed comprising elite event EE-GM3, said method comprising crossing the plant according to claim 11 with another soybean plant, and planting the seed comprising elite event EE-GM3 obtained from said cross.

24. A soybean product produced from the seed of claim 11, wherein said soybean product comprises elite event EE-GM3 and is soybean meal, ground seeds, soybean flour, or soybean flakes.

32. A method for producing a soybean plant tolerant to glyphosate and/or isoxaflutole herbicides, comprising introducing into the genome of said soybean plant elite event EE-GM3 of claim 11, and selecting a progeny plant tolerant to glyphosate and/or isoxaflutole, wherein said progeny plant comprises elite event EE-GM3.

33. The method of claim 32, wherein said introducing comprises crossing a first soybean plant lacking elite event EE-GM3 with a soybean plant comprising elite event EE-GM3, and selecting a progeny plant tolerant to glyphosate and/or isoxaflutole, wherein said progeny plant comprises elite event EE-GM3.

12. A soybean plant, plant cell, tissue, or seed, comprising in its genome elite event EE-GM3, wherein said event comprises a nucleic acid sequence of SEQ ID No. 2 and a nucleic acid sequence of SEQ ID No. 3, or the complement of said sequences.

34. A method for producing a soybean plant tolerant to glyphosate and/or isoxaflutole herbicides, comprising crossing a first soybean plant lacking elite event EE-GM3 with the soybean plant comprising elite event EE-GM3 of claim 12, and selecting a progeny plant tolerant to glyphosate and/or isoxaflutole, wherein said progeny plant comprises elite event EE-GM3.

51. A soybean product produced from the seed of claim 12, wherein said soybean product comprises elite event EE-GM3 and is soybean meal, ground seeds, soybean flour, or soybean flakes.

52. A method for controlling weeds in a field of the soybean plants or seeds of claim 12, comprising treating a field with an HPPD inhibitor herbicide, before said soybean plants are planted or the seeds are sown, followed by planting or sowing of said soybeans in that same pre-treated field.

53. A method for controlling weeds in a field of the soybean seeds of claim 12, comprising treating a field in which said seeds were sown, with an HPPD inhibitor herbicide before the soybean plants emerge but after the seeds are sown.

54. A method for controlling weeds in a field of the soybean plants of claim 12, comprising treating said plants with an HPPD inhibitor herbicide over the top of the soybean plants, which application can be together with, followed by or preceded by a treatment with glyphosate as post-emergent herbicide over the top of the plants.

13. A soybean plant, cell, tissue or seed, comprising in its genome elite event EE-GM3, wherein said event comprises a nucleic acid sequence of SEQ ID No. 2 from nucleotide 1431 to 1472 and a nucleic acid sequence of SEQ ID No. 3 from nucleotide 220 to 261, or the complement of said sequences.

35. A method for producing a soybean plant tolerant to glyphosate and/or isoxaflutole herbicides, comprising crossing a first soybean plant lacking elite event EE-GM3 with the soybean plant comprising elite event EE-GM3 of claim 13, and selecting a progeny plant tolerant to glyphosate and/or isoxaflutole, wherein said progeny plant comprises elite event EE-GM3.

14. A soybean plant, plant cell, tissue, or seed, comprising in its genome elite event EE-GM3, wherein said event comprises a nucleic acid sequence of SEQ ID No. 11 from nucleotide position 1452 to nucleotide position 16638 or the complement thereof.

25. A soybean product produced from the seed of claim 14, wherein said soybean product comprises elite event EE-GM3 and is soybean meal, ground seeds, soybean flour, or soybean flakes.

31. A method for producing a soybean plant tolerant to glyphosate and/or isoxaflutole herbicides, comprising crossing a first soybean plant lacking elite event EE-GM3 with the soybean plant comprising elite event EE-GM3 of claim 14, and selecting a progeny plant tolerant to glyphosate and/or isoxaflutole, wherein said progeny plant comprises elite event EE-GM3.

36. A method for producing a soybean plant tolerant to glyphosate and/or isoxaflutole herbicides, comprising crossing a first soybean plant lacking elite event EE-GM3 with the soybean plant of claim 14, and selecting a progeny plant tolerant to glyphosate and/or isoxaflutole, wherein said progeny plant comprises elite event EE-GM3.

50. A method for producing a soybean plant or seed comprising elite event EE-GM3, said method comprising crossing the plant according to claim 14 with another soybean plant, and planting the seed comprising elite event EE-GM3 obtained from said cross.

15. A soybean plant, plant cell, tissue, or seed, comprising in its genome elite event EE-GM3, wherein said event comprises a nucleic acid sequence comprising the complement thereof.

37. A method for producing a soybean plant tolerant to glyphosate and/or isoxaflutole herbicides, comprising crossing a first soybean plant lacking elite event EE-GM3 with the soybean plant of claim 15, and selecting a progeny plant tolerant to glyphosate and/or isoxaflutole, wherein said progeny plant comprises elite event EE-GM3.

42. A soybean plant, plant part, cell tissue, or seed comprising elite event EE-GM3.

43. A method for producing a soybean plant or seed comprising elite event EE-GM3, said method comprising crossing the plant according to claim 42 with another soybean plant, and planting the seed comprising elite event EE-GM3 obtained from said cross.

44. A soybean product produced from the seed of claim 42, wherein said soybean product comprises elite event EE-GM3 and is soybean meal, ground seeds, soybean flour, or soybean flakes.

45. A method for producing a soybean plant tolerant to glyphosate and/or isoxaflutole herbicides, comprising introducing into the genome of said soybean plant elite event EE-GM3 of claim 42, and selecting a progeny plant tolerant to glyphosate and/or isoxaflutole, wherein said progeny plant comprises elite event EE-GM3.

46. A method for controlling weeds in a field of the soybean plant of claim 42, comprising treating the field with an effective amount of an isoxaflutole-based herbicide, wherein said soybean plant is tolerant to said isoxaflutole-based herbicide.

47. A method for controlling weeds in a field of the soybean plants or seeds of claim 42, comprising treating a field with an HPPD inhibitor herbicide, before said soybean plants are planted or the seeds are sown, followed by planting or sowing of said soybeans in that same pre-treated field.

48. A method for controlling weeds in a field of the soybean seeds of claim 42, comprising treating a field in which said seeds were sown, with an HPPD inhibitor herbicide before the soybean plants emerge but after the seeds are sown.

49. A method for controlling weeds in a field of the soybean plants of claim 42, comprising treating said plants with an HPPD inhibitor herbicide over the top of the soybean plants, which application can be together with, followed by or preceded by a treatment with glyphosate as post-emergent herbicide over the top of the plants.