Systems and methods for multiple analyte detection

• US 8,597,590 B2
• Filed: 05/27/2010
• Issued: 12/03/2013
• Est. Priority Date: 04/26/2005
• Status: Active Grant

• Current Assignee: Applied Biosystems LLC (Thermo Fisher Scientific Incorporated)
• Original Assignee: Applied Biosystems LLC (Thermo Fisher Scientific Incorporated)
• Inventors: Yue, Min, Liu, David M., Roy, Joy, Chiang, Yuh-Min, Yang, Joon Mo, Lehto, Dennis, Vann, Charles S., Beard, Nigel P., Harding, Ian A., Van Camp, John R., Dromaretsky, Alexander, Ermakov, Sergey V., Oldham, Mark F., Shariati, Maryam, Ulmanella, Umberto
• Primary Examiner(s): MERKLING, SALLY ANNE
• Application Number:
  US12/789,363
• Publication Number:
  US 20110020179A1
• Time in Patent Office: 1,286 Days
• Field of Search: None
• US Class Current: 422/402
• CPC Class Codes: B01J 2219/00317 Microwell devices, i.e. having large numbers of wells
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01L 2200/0684 Venting, avoiding backpressure, avoid gas bubbles
• CPC Class Codes: B01L 2200/0689 Sealing
• CPC Class Codes: B01L 2200/16 Reagents, handling or storing thereof
• CPC Class Codes: B01L 2300/041 Connecting closures to device or container
• CPC Class Codes: B01L 2300/048 enabling gas exchange, e.g. vents
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2300/087 Multiple sequential chambers
• CPC Class Codes: B01L 2400/0481 squeezing of channels or chambers
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 2400/0655 pinch valves
• CPC Class Codes: B01L 2400/0694 vents used to stop and induce flow, backpressure valves
• CPC Class Codes: B01L 3/502707 characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: B01L 3/502723 characterised by venting arrangements
• CPC Class Codes: B01L 3/50853 with covers or lids
• CPC Class Codes: C12Q 1/6806 Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay C12Q1/6804 takes precedence
• CPC Class Codes: G01N 35/028 : having reaction cells in the form of microtitration plates
• CPC Class Codes: Y10T 436/2575 : Volumetric liquid transfer

Abstract

Systems and methods for multiple analyte detection include a system for distribution of a biological sample that includes a substrate, wherein the substrate includes a plurality of sample chambers, a sample introduction channel for each sample chamber, and a venting channel for each sample chamber. The system may further include a preloaded reagent contained in each sample chamber and configured for nucleic acid analysis of a biological sample that enters the substrate and a sealing instrument configured to be placed in contact with the substrate to seal each sample chamber so as to substantially prevent sample contained in each sample chamber from flowing out of each sample chamber. The substrate can be constructed of detection-compatible and assay-compatible materials.

Claims

1. A system for distribution of a biological sample, the system comprising:

a substrate, wherein the substrate comprises a main fluid supply channel connected to and fluidly communicating with a plurality of sample introduction channels, each sample introduction channel being connected to and fluidly communicating with a sample chamber, each sample chamber being connected to and fluidly communicating with a venting channel;

a preloaded reagent contained in each sample chamber and configured for nucleic acid analysis of a biological sample that enters the substrate; and

a sealing instrument, operable to individually isolate each sample chamber from each other so as to substantially prevent sample contained in each sample chamber from flowing out of each sample chamber,

wherein the sealing instrument comprises a plurality of protrusions selected from the group consisting of thermal transfer dies, substantially arc-shaped pins, circumferential disks and circular pins, each of the plurality of protrusions aligned to intersect and deform the sample introduction or venting channel of each sample chamber,

wherein the substrate is constructed of detection-compatible and assay-compatible materials.

2. The system of claim 1, wherein the sealing instrument is configured to be placed in contact with an exterior portion of the substrate.

3. The system of claim 1, wherein the sealing instrument is configured to be placed in at least one of pressure contact and thermal contact with the substrate.

4. The system of claim 1, wherein the sealing instrument is configured to seal the sample introduction channels and venting channels for each sample chamber.

5. The system of claim 1, wherein the sealing instrument is configured to be placed in contact with the substrate at least one of before a reaction process that occurs in the sample chambers and during a reaction process that occurs in the sample chambers.

6. The system of claim 5, wherein the sealing instrument is configured to be placed in contact with the substrate before a reaction process that occurs in the sample chambers to seal each sample chamber.

7. The system of claim 1, wherein the substrate further comprises a film layer and a base, and at least one venting mechanism configured to permit gas to escape the substrate.