Asymmetrically branched polymer conjugates and microarray assays

• US 8,597,653 B2
• Filed: 07/11/2010
• Issued: 12/03/2013
• Est. Priority Date: 11/21/2003
• Status: Active Grant

• Current Assignee: ANP Technologies, Inc.
• Original Assignee: ANP Technologies, Inc.
• Inventors: Yin, Ray, Qin, Dujie, Pan, Jing
• Primary Examiner(s): Choi, Ling
• Assistant Examiner(s): Mesh, Gennadiy
• Application Number:
  US12/833,988
• Publication Number:
  US 20110053243A1
• Time in Patent Office: 1,241 Days
• Field of Search: 525/420
• US Class Current: 424/178.1
• CPC Class Codes: A61K 47/6949 inclusion complexes, e.g. clathrates, cavitates or fullerenes
• CPC Class Codes: A61K 49/0002 General or multifunctional contrast agents, e.g. chelated agents
• CPC Class Codes: B82Y 10/00 Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: B82Y 5/00 Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
• CPC Class Codes: G01N 33/5308 for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
• CPC Class Codes: Y10S 435/975 Kit
• CPC Class Codes: Y10T 436/11 Automated chemical analysis

Abstract

A conjugate of a modified randomly branched asymmetric polymer without a core and a member of a binding pair is described. The modified randomly branched asymmetric polymer can contain chain branches, terminal branches or both. The modified randomly branched asymmetric polymer can contain random asymmetric branches or random asymmetric junctions. The binding pair can be an antibody, antigen or a ligand.

Claims

1. A conjugate comprising:

(a) a modified randomly branched polyethyleneimine or polyoxazoline polymer without a core having a molecular weight of from about 500 to about 1,000,000 and comprising a functional group for branch formation, wherein said functional group is selected from the group consisting of —

NH₂, —

NHR, —

COOR, —

COOH and a combination thereof, wherein R is an aliphatic or aromatic group; and

wherein said functional group for branch formation is produced by reaction with a methyl acrylate, an ethylenediamine or both; and

(b) a member of a binding pair,

wherein said conjugate is used to detect a target molecule.

2. The conjugate of claim 1, wherein said modified polyoxazoline comprises a modified poly (2-methyoxazoline) or a modified poly (2-ethyloxazoline).

3. The conjugate of claim 1, wherein said binding pair comprises a polynucleotide, an antibody, an antigen-binding portion thereof, an antigen or an epitope-containing portion thereof.

4. The conjugate of claim 1, wherein said member of said binding pair binds a polymer, a hormone, a neurotransmitter, a pathogen, a toxin or a drug.

5. The conjugate of claim 4, wherein said polymer comprises a polypeptide, a polysaccharide or a polynucleotide.

6. The conjugate of claim 5, wherein said polypeptide comprises an enzyme.

7. The conjugate of claim 5, wherein said polypeptide comprises an antibody, an antigen-binding portion thereof, an antigen or an epitope-containing portion thereof.

8. The conjugate of claim 5, wherein said polynucleotide comprises a DNA or an RNA.

9. The conjugate of claim 7, wherein said antigen-binding polypeptide comprises an immunoglobulin or antigen-binding portion thereof.

10. The conjugate of claim 8, wherein said RNA comprises an RNAi.

11. The conjugate of claim 4, wherein said polymer comprises a polyethylene glycol.

12. The conjugate of claim 1, wherein said member of said binding pair comprises an avidin, an antibody which binds a drug, or both, wherein said drug comprises a digoxin.

13. The conjugate of claim 1, wherein said member of said binding pair comprises a biotin, an antibody which binds a drug, or both, wherein said drug comprises a digoxin.

14. The conjugate of claim 1, wherein said member of said binding pair comprises streptavidin.

15. The conjugate of claim 1, wherein said member of said binding pair comprises colloidal gold.

16. The conjugate of claim 4, wherein said pathogen comprises a bacterium, a spore, a parasite or a virus.