Systems and methods for manufacturing quantum dot-doped polymer microbeads
First Claim
1. A method of forming microbeads comprising the steps of:
- (a) flowing a polymer stream of a polymer solution into an interior chamber of a flow focusing body, with the polymer solution comprising particles and a polymer dissolved in a solvent;
(b) flowing a focusing fluid into the chamber, with the focusing fluid capable of reacting with the polymer solution to form functional groups adapted to bind with biorecognition molecules;
(c) directing the focusing fluid into contact with the polymer stream in the chamber, so as to focus the polymer stream;
(d) flowing the focusing fluid and the polymer stream focused thereby, as a single flow stream, out from the chamber; and
(e) allowing detachment of pendant droplets from a leading end portion of the single flow stream so as to form the microbeads;
wherein each of the microbeads binds an identifiable set of the particles; and
wherein the focusing fluid reacts with the polymer solution to form one or more of the functional groups at a surface of each of the microbeads, such that the microbeads are adapted to bind with the biorecognition molecules.
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Abstract
In a method and system for forming microbeads, a polymer solution includes particles and a polymer dissolved in a solvent. A stream of the polymer solution flows into a chamber. A focusing fluid contacts and focuses the polymer stream in the chamber. The focusing fluid and the focused polymer stream flow, as a single flow stream, out from the chamber. Pendant droplets detach from a leading end of the single flow stream to form the microbeads. The focusing fluid reacts with the polymer solution to form functional groups at a surface of the microbeads for binding with biorecognition molecules. In the system, a flow focusing apparatus includes a flow focusing body shaped to define the chamber. Microbeads formed according to the methods and systems are also disclosed.
170 Citations
59 Claims
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1. A method of forming microbeads comprising the steps of:
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(a) flowing a polymer stream of a polymer solution into an interior chamber of a flow focusing body, with the polymer solution comprising particles and a polymer dissolved in a solvent; (b) flowing a focusing fluid into the chamber, with the focusing fluid capable of reacting with the polymer solution to form functional groups adapted to bind with biorecognition molecules; (c) directing the focusing fluid into contact with the polymer stream in the chamber, so as to focus the polymer stream; (d) flowing the focusing fluid and the polymer stream focused thereby, as a single flow stream, out from the chamber; and (e) allowing detachment of pendant droplets from a leading end portion of the single flow stream so as to form the microbeads; wherein each of the microbeads binds an identifiable set of the particles; and wherein the focusing fluid reacts with the polymer solution to form one or more of the functional groups at a surface of each of the microbeads, such that the microbeads are adapted to bind with the biorecognition molecules. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30)
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31. A system for forming microbeads comprising:
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(a) a polymer solution comprising particles and a polymer dissolved in a solvent; (b) a focusing fluid capable of reacting with the polymer solution to form functional groups adapted to bind with biorecognition molecules; (c) a flow focusing apparatus comprising a flow focusing body shaped to define an interior chamber and an exit opening, with the chamber operatively receiving the focusing fluid and a polymer stream of the polymer solution, with the focusing fluid operatively contacting and focusing the polymer stream in the chamber, so as to focus the polymer stream, and with the focusing fluid and the polymer stream focused thereby operatively flowing, as a single flow stream out from the chamber through the exit opening; wherein pendant droplets operatively detach from a leading end portion of the single flow stream so as to form the microbeads; wherein each of the microbeads binds an identifiable set of the particles, and wherein the focusing fluid operatively reacts with the polymer solution to form one or more of the functional groups at a surface of each of the microbeads, such that the microbeads are adapted to bind with the biorecognition molecules. - View Dependent Claims (32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59)
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Specification