Modified nucleotides
First Claim
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1. A method of converting a compound of formula R—
- O-allyl, R2N(allyl), RNH(allyl), RN(allyl)2 or R—
S-allyl to a corresponding compound in which the allyl group is removed and replaced by hydrogen, said method comprising the steps of reacting a compound of formula R—
O-allyl, R2N(allyl), RNH(allyl), RN(allyl)2 or R—
S-allyl in aqueous solution with a transition metal comprising a transition metal and one or more ligands selected from the group comprising water-soluble phosphine and water-soluble nitrogen-containing phosphine ligands, wherein the or each R is a water-soluble biological molecule, which is part of a nucleoside, a nucleotide or a polynucleotide molecule, wherein said nucleoside, nucleotide or polynucleotide further comprises a detectable label linked to the base thereof by a cleavable or non-cleavable linker.
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Abstract
Provided herein is a method for replacing the allyl group in a compound of formula R—O-allyl, R2N(allyl), RNH(allyl), RN(allyl)2 or R—S-allyl with a hydrogen, using a transition metal and one or more phosphine ligands.
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Citations
15 Claims
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1. A method of converting a compound of formula R—
- O-allyl, R2N(allyl), RNH(allyl), RN(allyl)2 or R—
S-allyl to a corresponding compound in which the allyl group is removed and replaced by hydrogen, said method comprising the steps of reacting a compound of formula R—
O-allyl, R2N(allyl), RNH(allyl), RN(allyl)2 or R—
S-allyl in aqueous solution with a transition metal comprising a transition metal and one or more ligands selected from the group comprising water-soluble phosphine and water-soluble nitrogen-containing phosphine ligands, wherein the or each R is a water-soluble biological molecule, which is part of a nucleoside, a nucleotide or a polynucleotide molecule, wherein said nucleoside, nucleotide or polynucleotide further comprises a detectable label linked to the base thereof by a cleavable or non-cleavable linker. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
- O-allyl, R2N(allyl), RNH(allyl), RN(allyl)2 or R—
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11. A method of converting a compound of formula R—
- O-allyl to a corresponding compound in which the allyl group is removed and replaced by hydrogen, said method comprising the steps of reacting a compound of formula R—
O-allyl in aqueous solution with a transition metal comprising a transition metal and one or more ligands selected from the group comprising water-soluble phosphine and water-soluble nitrogen-containing phosphine ligands, wherein the R is a water-soluble biological molecule, which is part of a nucleoside, a nucleotide or a polynucleotide molecule, wherein said nucleoside, nucleotide or polynucleotide further comprises a detectable label linked to the base thereof by a cleavable or non-cleavable linker.
- O-allyl to a corresponding compound in which the allyl group is removed and replaced by hydrogen, said method comprising the steps of reacting a compound of formula R—
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12. A method of converting a compound of formula R2N(allyl) to a corresponding compound in which the allyl group is removed and replaced by hydrogen, said method comprising the steps of reacting a compound of formula R2N(allyl) in aqueous solution with a transition metal comprising a transition metal and one or more ligands selected from the group comprising water-soluble phosphine and water-soluble nitrogen-containing phosphine ligands, wherein each R is independently a water-soluble biological molecule, which is part of a nucleoside, a nucleotide or a polynucleotide molecule, wherein said nucleoside, nucleotide or polynucleotide further comprises a detectable label linked to the base thereof by a cleavable or non-cleavable linker.
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13. A method of converting a compound of formula RNH(allyl) to a corresponding compound in which the allyl group is removed and replaced by hydrogen, said method comprising the steps of reacting a compound of formula RNH(allyl) in aqueous solution with a transition metal comprising a transition metal and one or more ligands selected from the group comprising water-soluble phosphine and water-soluble nitrogen-containing phosphine ligands, wherein the R is a water-soluble biological molecule, which is part of a nucleoside, a nucleotide or a polynucleotide molecule, wherein said nucleoside, nucleotide or polynucleotide further comprises a detectable label linked to the base thereof by a cleavable or non-cleavable linker.
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14. A method of converting a compound of formula RN(allyl)2 to a corresponding compound in which the allyl group is removed and replaced by hydrogen, said method comprising the steps of reacting a compound of formula RN(allyl)2 in aqueous solution with a transition metal comprising a transition metal and one or more ligands selected from the group comprising water-soluble phosphine and water-soluble nitrogen-containing phosphine ligands, wherein the R is a water-soluble biological molecule, which is part of a nucleoside, a nucleotide or a polynucleotide molecule, wherein said nucleoside, nucleotide or polynucleotide further comprises a detectable label linked to the base thereof by a cleavable or non-cleavable linker.
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15. A method of converting a compound of formula R—
- S-allyl to a corresponding compound in which the allyl group is removed and replaced by hydrogen, said method comprising the steps of reacting a compound of formula R—
S-allyl in aqueous solution with a transition metal comprising a transition metal and one or more ligands selected from the group comprising water-soluble phosphine and water-soluble nitrogen-containing phosphine ligands, wherein the R is a water-soluble biological molecule, which is part of a nucleoside, a nucleotide or a polynucleotide molecule, wherein said nucleoside, nucleotide or polynucleotide further comprises a detectable label linked to the base thereof by a cleavable or non-cleavable linker.
- S-allyl to a corresponding compound in which the allyl group is removed and replaced by hydrogen, said method comprising the steps of reacting a compound of formula R—
Specification